Kemampuan Prospektif Fusan FP4 dalam Memproduksi Inulinase dan Profil Pertumbuhannya

https://doi.org/10.31957/jbp.105

Authors

  • Wijanarka Wijanarka Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro
  • Ika Anggraini Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang, Indonesia

Abstract

Fusan FP4 is one type fused materials derived from intraspecific fusion of Pichia manshurca. The fused materials have superior capabilities compared with other materials, especially in producing inulinase enzyme (E.C. 3.2.1.7). The aims of the study are to determine the production of the enzyme inulinase of fusan FP4 and the growth profile formed. Growth can be detected by the addition of size and cell division. Growth measurements of fusan FP4 was carried out for 24 hours and observation and sampling was done every 6 hours for 24 hours. Observations of growth are conducted qualitatively by using a spectrophotometer, while the production of inulinase done using DNS method. The results of this study showed that the highest inulinase production was 1.948 U/ml and occurred at log phase with 6 hours of incubation time (t6) to 12 hours (t12). 

Key words: inulinase, Fusan FP4, log phase, P. manshurca.

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Author Biographies

Wijanarka Wijanarka, Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Departemen Biologi, Fakultas Sains dan Matematika, Undip

Ika Anggraini, Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang, Indonesia

Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro, Semarang, Indonesia

References

Danial, E.N, N.O.A. Ayaz, and H.S.O. Alnahdi. 2015. Production of inulinase by free and immobilized cells of Penicillium funiculosum. Braz Arch Bio Technol. 58(4): 36.

Karatop. R., and F. Sanal. 2013. A potential resource in fructose production from inulin: Aspergillus wentii inulinase. Journal of Cell and Molecular Biology. 11(1&2): 21-28.

Khurtzman, C.P. 2000. Four new yeasts in the Pichia anomala clade. Journal of Systematic and Evolutionary Microbiology. 50: 395-404.

Laowklom, N, R. Chantanapan, and P. Pinphanichakarn. 2012. Production, purification and characterization of inulin-ase from a newly isolated Streptomyces sp. CP01. Natural Resource. 3: 137-144.

Leva, L.A.G., S.R. Hughes, J.C.L. Nunez, J.M. Jarodsky, A. Erickson, M.R. Lindquist, L.J. Cox, K.M. Bischoff, E.C. Hoecker, S. Liu, N. Qureshi, and M.A. Jones. 2016. Growth ethanol production and inulinase activity on various inulin substrate by mutant Kluveromyces marxianus Strain NRRL Y-50798 and NRRL Y-50799. J Ind Microbiol Technol. 43: 927-939.

Mangunwidjaja, D, M. Rahayuningsih, dan R. Suparwati. 2014. Pengaruh konsentrasi enzim dan waktu hidrolisis enzimatis terhadap mutu frukto-oligosakarida dari inulin umbi Dahlia (Dahlia pinnata). E-Jurnal Agroindustri Indonesia 3(1): 189-199.

Pessoni, R.A.B, M.R. Braga, and R.D.C.L.F. Ribeiro. 2007. Purification and properties of exo-Inulinase from penicillium janczewskii growing on distinct carbon sources. Mycologia 99 (4): 493-503.

Roslenawati, H. P. Kusumaningrum, dan S. Pujiyanto. 2014. Analisis fusan hasil fusi protoplas intraspesies Pichia manshurica DUCC-015. Jurnal Sains dan Matematika 22(1): 7-14.

Santos, A.M.P. and F. Maugeri. 007. Synthesis of fructooligosaccharides from sucrose using inulinase from Kluyveromyces marxianus. J. Food Technol and Biotechnol. 45(2): 181-186.

Sari, S.L.A, dan H. Hartiko. 2005. Aktivitas inulinase jamur yang diisolasi dari tanah sekitar umbi dahlia (Dahlia pinnata cav). Biosmart. 7(5): 1-5.

Waluyo, L. 2004. Mikrobiologi umum. Universitas Muhammadiyah Press. Malang.

Wijanarka, R.S Ferniah, dan Salamah. 2004. Produksi inulinase Pichia alni DUCC-W4 pada tepung umbi dahlia (Dahlia Variabilis Willd) dengan variasi konsentrasi ammonium nitrat dan waktu inkubasi. Bioma. 10(2): 58-64.

Wijanarka, E. Kusdiyantini, dan Hermin. 2009. Identifikasi dan karakterisasi isolat 23 Ducc serta kemampuannya dalam memproduksi enzim inulinase. Biosfera. 26(3): 108-114.

Wijanarka, E.S. Soetarto, K. Dewi, dan A. Indrianto. 2013. Aktivitas inulinase oleh Pichia manshurica dan Fusan F4 pada fermentasi batch dengan umbi dahlia (Dahlia sp) sebagai substrat. Reaktor. 14(3): 187-192.

Wijanarka, E.S. Soetarto, K. Dewi, dan A. Indrianto. 2013. Kinetika pertumbuhan dan produksi inulinase Fusan 7. Bioma. 15(2): 53-57.

Xia, Li. A, L.Z. Guo, and W.D. Lu. 2012. Alkaline inulinase production by a newly isolated bacterium Marinimicrobium sp. LS-A18 and inulin hidrolysis by the enzyme. World J. Microbiol. Biotechnol. 28: 81-89.

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Published

2018-05-14

Issue

Vol. 9 No. 1 (2017)

Section

Research Articles

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