Pengendalian Infeksi Bakteri Aeromonas hydrophila pada Ikan Nila (Oreochromis niloticus) dengan Ekstrak Rimpang Lengkuas Merah (Alpinia purpurata)
DOI:
https://doi.org/10.31957/jbp.110Abstract
MAS diseases (Motile Aeromonas Septicaemia) is caused by the bacterium Aeromonas hydrophila. One of the natural ingredients that can be used as an alternative to inhibit bacterial activity is the extract of red galangal rhizome (Alpinia purpurata). Active substances possessed of this material have potential as antibacterial. The purpose of this study is to determine the effectiveness of red galangal rhizome extract in the control of MAS disease in tilapia. The type of this research is experimental research in Completely Random Design (RAL) with 6 treatments, which are positive control, negative control, 250, 500, 750, and 1000 ppm. The result of the research on the activity of the galangal extract bacteria on A. hydrophila obtained the widest clear zone at 1000 ppm constipation, which is 9.7625 mm. The treatment has been conducted by immersion of Tilapia fish into the extracted red galangan rgizomes for 5 minutes, then it was put into aquarium for maintenance for 7 days. 0.1 ml A. hydrophila (108 cfu/ml) was injected to Tilapia. The result of the research on antibacterial shoes the wides clear zone at 1000 ppm with constipation, which is 9.7625 mm and the effectiveness test of red galangal extract on tilapia infected by A. hydrophila bacteria experience (ulcer) healing after 7 days with the concentration of rhizome extract of galangal 1000 ppm.Â
Key words:Â red galangal rhizome extract, A. hydrophila, A. purpurata.
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References
Boyd, C.E. 1982. Water quality in ponds for aquaculture. International Centre for Aquaculture Experiment Station. Auburn University. Alabama.
Chopra, A.K., X.I. Xu, D. Ribardo, M. Gonzales, K. Kuhl, J.W. Peterson, and C.W. Huston. 2000. The cytoxic enterotoxin of Aeromonas hydrophila induce proinflamantory cytokine production and activates arachidonic acid metabolism in machrophage. Infection and Immunity. 68(5): 2808-2818.
Cavalieri, S.J., I.O. Rankin, R.J. Harbeck, R.S. Sauter, Y.S. McCater, S.E. Sharp, J.H. Ortez, and C.A. Spiegel. 2005. Manual of antimicrobial susceptibility testing. American Society for Microbiology. USA.
Dinas Kelautan dan Perikanan Kabupaten Jayapura. 2012. Laporan Tahunan Dinas Kelautan dan Perikanan Kabupaten Jayapura Tahun 2012.
Hossain, M.D., M.K. Hossain, M.H. Rahman, A. Akter, and D.A. Khanom. 2008. Prevalence of ectoparasites of carp fingerlings at Santaher, Bogra. Universal Journal of Zooog. 27: 17-19.
Indrayani, E., K.H. Nitimulyo, S. Hadisusanto dan Rustadi. 2015. Peta batimetri Danau Sentani Papua. Depik. 4(3): 116-120.
Kabata, Z. 1985. Parasites and disease of fish cultured in the tropics. Taylor and Fancis Press. London.
Khairuman dan K. Amri. 2008. Buku pintar budidaya 15 ikan konsumsi. PT Agromedia Pustaka. Jakarta.
Kordi, M. dan H. Gufron. 2010. Nikmat rasanya, nikmat untungnya pintar budi daya ikan di tambak secara intensif. Yogyakarta.
Kumar, S., and A.K. Pandey. 2013. Chemostry and biological activities of flavonoids: an overview. The Scientific World Journal. ID162750: 1-16.
Laith, A.R., and M. Najiah. 2013. Aeromonas hydrophila: Antimicrobial susceptibility and histopathology of isolates from diseased catfish Clarias gariepinus (Burchhell). J Aquac Res Development. 5(2): 1-7.
Mulia, D.S. 2003. Pengaruh vaksin debris sel Aeromonas hydrophila dengan kombinasi cara vaksinasi dan booster terhadap respons imun dan tingkat perlindungan relatif pada lele dumbo (Clarias gariepinus Burchell). [Tesis]. Universitas Gajah Mada. Yogyakarta.
Noventi, W., dan C. Novita. 2016. Potensi ekstrak daun sirih hijau (Piper betle L.) sebagai alternatif terapi Acne vulgaris. Majority. 5(1): 140-145.
Parwata, O.A. 2008. Isolasi dan uji aktifitas antibakteri minyak atsiri dari rimpang lengkuas (Alpinia galanga). Jurnal Kimia. 2(2): 100-104.
Pratama, M.R. 2005. Pengaruh ekstrak serbuk kayu siwak (Salvadora persica) terhadap pertumbuhan bakteri Streptococcus mutans dan Staphylococcus aureus dengan Metode Difusi Agar. [Skripsi]. Institut Pertanian Bogor Bogor.
Pratiwi, I. 2009. Uji antibakteri ekstrak kasar daun Acalypha indica terhadap bakteri Salmonella choleraesuis dan Salmonella typhimurium. [Skripsi]. Universitas Sebelas Maret (UNS). Surakarta.
Rahman, M.F. 2008. Potensi antibakteri ekstrak daun papaya pada ikan gurami yang diinfeksi bakteri Aeromonas hydrophila. [Skripsi]. IPB, Bogor.
Redha, A. 2010. Flavonoid: Struktur, sifat antioksidatif dan peranannya dalam sistem biologis. Jurnal Belian. 9(2): 196-202.
Siniwoko, E.D. 2013. Budidaya dan bisnis ikan nila untuk pemula. Dafa Publising. Surabaya.
Sumayani, K. Rahayu, dan C. Yudi. 2008. Daya antibakteri perasan rimpang lengkuas (Alpinia galanga) dengan konsentrasi berbeda terhadap pertumbuhan Aeromonas hydrophila Secara in vitro. Berkalah Ilmiah Perikanan. 3(1): 83-87.
Taufiq, S., Y. Umi, dan H. Siti. 2015. Uji aktivitas antibakteri ekstrak etanol biji buah pepaya (Carica papaya) terhadap Escherichia coli dan Salmonela typhi. Prosiding Penelitian SPeSIA UNISBA.
Wattimena, J.R., N.C. Sugiarso, M.B. Widianto, E.Y. Sukandar, A.A. Soemarji, dan A.R. Setiadi, A.R. 1991. Farmakologi dan terapi antibiotik. Universitas Gadjah Mada. Yogyakarta.
