Antiproliferatif Ekstrak Metanol Daun Dianella nemorosa Lam. (Liliaceae) terhadap Sel Kanker MKN45 dengan Menggunakan Metode WST-1

Authors

  • Aditya K. Karim Jurusan Biologi FMIPA Universitas Cenderawasih, Jayapura–Papua
  • Sismindari Sismindari Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta

DOI:

https://doi.org/10.31957/jbp.548

Abstract

Dianella nemorosa Lam. was known containing alkaloids, terpenoid, phenolic compouds and tanin. Antiproliferative effect of D. nemorosa leaves methanol exctract, which demonstrated to have an in vitro cytotoxic effect on cancer cell line. The aim of research was examined the effect antiproliferative methanol extract of D. nemorosa leaves against MKN45 (gastric cancer) cell line. Leaves powder extracted using methanol. Antiproliferative effect was determined by Cell Proliferation Reagents WST-1 ((2-(4-iodophenyl)-3-(4-nitro-phenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt). Test for 1h, 2h, and 4h after incubated for 72h was done. The result of this research showed that methanol exctract from D. nemorosa possessed remarkable no had antiproliferative activity against MKN45 cell line. The result indicated methanol extract of D. nemorosa leaves selective inhibitory effect or antiproliferative against cell line. 

Key words: Dianella nemorosa, Antiproliferative, MKN 45, and WST-1

Downloads

Download data is not yet available.

Author Biographies

Aditya K. Karim, Jurusan Biologi FMIPA Universitas Cenderawasih, Jayapura–Papua

Jurusan Biologi FMIPA Universitas Cenderawasih, Jayapura–Papua

Sismindari Sismindari, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta

Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta

References

Alberts, S.R., A.Cervantes, and C.J. van de Velde. 2003. Gastric cancer: Epidemiology, pathology and treatment. Ann.Oncol, 14 (2):31-36.

Arunporn, I., J.H. Peter, P.J. Eno-Amooquaye, H.S., Burker, Julia, and R. Amala. 2004. In vitro cytotoxic activity of Thai medicinal plant used traditionaly to treat cancer. J. Ethnopharmacol. 33-38.

Athima, S., I. Arunporn, D. Chawaboon, W. Chatchai, K. Niwat, and R. Pranee. 2009. Cytotoxic activity of Thai medicinal plant for cancer treatment. J. Sci. Technol. 27(2): 470-478.

Ahmad, B. 2010. Antioxidant activity and phenolic compounds from Colchicum luteum Baker (Liliaceae). African J. Biotech. 9(35): 5762-5766.

Byrne, L.T., S.M. Colegate, P.R. Dorling and C.R. Huxtable. 1987. Imbrica-tonol, a naphthol naphthoquinone dimer isolated from Stypandra imbricata and Dianella revolute. Australian J.Chem. 40(7): 1315– 1320.

Babula, P., V. Adam, L. Havel and R. Kizek. 2009. Noteworthy secondary metabolites naphthoquinones: Their occurrence pharmacological properties and analysis. Curr. Pharm. Analys. 5: 47-68.

Bozcuk, H., M. Ozdogan, O. Aykurt, F. Topcuoglu, H. Ozturk, D. Ekinci, A. Karadeniz., A. Mutlu, and D. Burgucu. 2011. Urginea maritima (L.) Baker (Liliaceae) extract induces more cytotoxicity than standard chemotherapeutics in the A549 Non-small Cell Lung Cancer (NSCLC) cell line. Turk J Med Sci. 41(1): 101-108.

Colegate, S.M., P.R. Dorling, and C.R. Huxtable. 1986. Dianellidin, stypandrol and dianellinone: An oxidation-related series from Dianella revolute. Phytochemstry. 25(5): 1245-1247.

Chee, Y.C., Kit, L.C., Koichi, T., and Hideji, I. 2001. Cytotoxic Activity Typhonium flagelliforme (Araceae). Phytother. Res. 15: 260-262.

Chawla, A., P. Chawla, and R.C.R Mangalesh. 2011. Asparagus racemosus (Willd): Biological activities and its active principles. Indo-Global J. Pharmac. Scie. l(2): 113-120.

Doyle, A., and J.B. Griffiths. 2000. Cell and tissue culture for medical research. John Willey and Sons.Ltd. New York. pp: 47-50.

Francoeur, A.M., and A. Assalian. 1996. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochem. (3): 19-25.

Feng, R.H., Z.G. Zhu, J.F. Li, B.Y. Liu, M. Yan, H.R. Yin, and Y.Z. Lin. 2002. Inhibition of human telomerase in MKN-45 cell line by antisense hTR expression vector induces cell apoptosis and growth arrest. World. J. Gastroenterol. 8(3): 436-440.

Hamburger, M., and Hostettman. 1991. Bioaktivity in plan: The link between phtyochemestry and medicine. Phytocehemstry. 30: 3864-3874.

Hohenberger, P., and S. Gretschel. 2003. Gastric cancer. Lancet, 362: 305-315.

Ikeguchi, M., J. Liu, and N. Kaibara. 2002. Expression of survivin mRNA and protein in gastric cancer cell line (MKN-45) during cisplatin treatment. Apoptosis. 7: 23–29.

Kelley, J.R, and J.M Duggan. 2003. Gastric cancer epidemiology and risk factors. J.Clin. Epidemiol. 56: 1-9.

Lojanapiwatna, V., C.Kovit, S. Kanchana, and Pichaet. 1982. Chemical constituents of Dianella ensifolia. J. Sci. Soc. Thailand. 8: 95-102.

Nurhanan, M.Y., O.Asiah, A. Mohd Ilham, M.M. Siti Syarifah, I. Norhayati, and H. L. Sahira. 2008. Anti-proliferative activities of 32 Malaysian plant species in breast cancer cell lines. J. Tropic. Forest Scien. 20: 77–81.

Roche, 2007. Cell proliferation reagent WST-1. Colorimetric assay (WST-1 based) for the non radioactive quantification of cell proliferation, cell viability and cytotoxicity. Roche Diagnostics GmbH. Roche Applied Science. Germany. Version October 2007: pp 1-4.

Sun, X.D., R. Mu, Y.S. Zhou, X.D. Dai, Y.L. Qiao, S.W. Zhang, F.X.M. Hang, J. Sun, L.D. Li, and F.Z. Lu. 2002. 1990-1992 Mortality of stomach cancer in China. Zhonghua Zhongliu Zazhi. 24: 4-8.

Srinivas, P., G. Gopinath, A. Banerji, A. Dinakar, and G. Srinivas. 2004. Plumbagin induces reactive oxygen species, which mediate apoptosis in human cervical cancer cells. Mol. Carcino genesis. 40: 201-211.

Tsunemitsu, Y., S. Kagawa, N. Tokunaga, S. Otani, T. Umeoka, J.A. Roth, B. Fang, N. Tanaka, and T. Fujiwara. 2004. Molecular therapy for peritoneal dissemination of xenotransplanted human MKN-45 gastric cancer cells with adenovirus mediated bax gene transfer. Gut. 53: 554-560.

Takahira, K., M. Sano, H. Arai, and H. Hanai. 2004. Apoptosis of gastric cancer cell line MKN45 by photodynamic treatment with photofrin. Lasers in Medical Science. 19: 89–94.

Ushijima, T., and M. Sasako. 2004. Focus on gastric cancer. Cancer Cell. 5: 121-125.

Vala, M.H., J. Asgarpanah, M.H. Hedayati, J. Shirali, and F.B. Bejestani. 2011. Antibacterial and cytotoxic activity of Eremurus persicus (Jaub and Spach) Boiss. African. J. Microbiol. Res. 5(16): 2349-2352.

Williams, L.A.D., J. Conrad, B. Vogler, H. Rösner, R.B.R. Porter, W. Setzer, E.N. Barton, H.G. Levy, S. Mika, I. Klaiber, J.P. Kurunziza, and W. Kraus. 2007. In vitro antiproliferation/cytotoxic activity of epingaione and its derivatives on the human SH-SY5Y neuroblastoma and TE-671 sarcoma cells. West Indian. Med. J. 56 (1): 5-10.

Wang, C.C., Y.M. Chiang, S.C. Sung, Y.L. Hsu, J.K. Chang, and P.L. Kuo. 2008. Plumbagin induces cell cycle arrest and apoptosis through reactive oxygen species/c-Jun N-terminal kinase pathways in human melanoma A375.S2 cells. Cancer Lett. 18;259(1): 82-98.

Wahyuningsih, M.S.H., Sismindari, dan Murti, Y.B. 2009. Potensi Herbal Terpilih Sebagai Agen Antikanker Yang Spesifik. Laporan Hibah Klaster, Klaster Kesehatan dan Kedokteran, Universitas Gadjah Mada.

Xiao, D.C, Y. Wen Cai, and C.Chun Tao. 2000. A new enolate furostanoside from Asparagus Filicinus. Chinese Chemical Letters. 11(9): 793−794.

Yoshioka, S., S. Tatebe, N. Tokuyasu, K. Taniguchi, Y. Ogami, O. Yamamoto, K. Ashida, Y. Gomyo, A. Akira Kondo, S. Tsujitani, and M. Ikeguchi. 2005. The PI3K-Akt pathway in SN-38-induced apoptosis in human gastric cancer cell lines. Yonago Acta Medica. 48: 7-16.

Yu, Z.H., Wei, P.K., Xu, Lu., Qin, Z.F., and Shi, J. 2006. Anticancer Effect of Jinlongshe Granules on In Situ-Transplanted Human MKN-45 Gastric Cancer in Nude Mice and Xenografted Sarcoma 180 in Kunming Mice and Its Mechanism. World. J. Gastroenterol, 12(18): 2890-2894.

Zullies, I., E.N. Agung, dan W. Winda. 2006. Efek ekstrak etanol daun Erythrina fusca Lour (cangkring) terhadap penekanan ekspresi enzim siklooksigenase-2 pada kultur sel raji. Majalah Farmasi Indonesia. 17(2): 85-90.

Zullies, I., E.N. Agung, dan A. Widyah. 2007. Penekanan ekspresi COX-2 pada kultur sel raji oleh ekstrak kloroform daun cangkring (Erythrina fusca Lour.). Majalah Obat Tradisional. 11(39):19-23.

Downloads

Published

2018-10-20

Issue

Section

Research Articles