Karakterisasi Mikrobia Rizosfer asal Tanaman Ginseng Jawa (Talinum triangulare) berdasarkan Gen Ribosomal 16S rRNA dan 18S rRNA

Authors

  • Alimuddin Ali Jurusan Biologi FMIPA Universitas Negeri Makassar
  • Herlina Rante Fakultas Farmasi, Universitas Hasanuddin, Makassar

DOI:

https://doi.org/10.31957/jbp.552

Abstract

The rhizosphere is a biologically active zone of the soil around plant roots that contains soil-borne microbes including bacteria and fungi.  The microbes were isolated from rhizosphere soil roots of Java ginseng. The population of microbes was estimated by plate count method. The isolates were identified based on a great variety of morphological, and cultural characteristics. The total of rhizosphere soil microbe population were  20.91(106 cfu.g−1soils) and showed that 12 isolates of bacteria, 15 isolates of actinomycetes, and 10 isolates of fungi which were found in all of soil samples. The molecular analysis of the ribosomal genes showed that the bacterial isolate, actinomycetes and fungi were closely related to of Staphylococcus sp. DGM  (JF923460), Streptomyces avidinii (EU593640) and fungi Aspergillus niger (HQ379853), respectively. 

Key words: rhizosphere, Java ginseng, 16S rRNA gene, 18S rRNA gene

Downloads

Download data is not yet available.

Author Biographies

Alimuddin Ali, Jurusan Biologi FMIPA Universitas Negeri Makassar

Jurusan Biologi FMIPA Universitas Negeri Makassar 

Herlina Rante, Fakultas Farmasi, Universitas Hasanuddin, Makassar

Fakultas Farmasi, Universitas Hasanuddin, Makassar

References

Berg, G., K. Opelt, C. Zachow, J. Lottmann, G.C. Monika, and K. Smalla. 2006. The rhizosphere selecton bacteria antagonistic towards the pathogenic fungus Verticillium di¡ers depending on plant species and site. FEMS Microbiol Ecol. 56: 250–261.

Chun, J. 1999. PHYDIT (The Phylogenetic Editor) Version 3.0. User’ Manual.

Felske, A., A. Wolterink, R. Lis, and A.D.L. Akkermans. 1998. Phylogeny of the main bacterial 16S rRNA sequences in a Drentse grassland soils (The Netherlands). Appl Environ Microbiol. 54: 871–879.

Hahn, D., A. Nickel and J. Dawson. 1999. Assessing Frankia populations in plants and soil using molecular methods. FEMS Microbiol Ecol. 29: 215-227.

Getha, K. and S. Vikinesway. 2005. Evaluation of Streptomyces sp. Strain g 10 for suppression of Fusarium wilt and rhizosphere colonization in pot-grown banana plantlets. J. Ind Microbiol Biotechnol 32(1): 24–32.

Grayston, S.J., D. Vaughan and D. Jones. 1996. Rhizosphere carbon flow in trees, in comparison with annual plants: the importance of root exudation and its impact on microbial activity and nutrient availability. Appl Soil Ecol. 5: 29–56.

Karthikeyan, B., A.C. Jaleel, G.M.A. Lakshmanan, and M. Deiveekasundaram. 2008. Studies on rhizosphere microbial diversity of some commercially important medicinal plants. Colloids and Surfaces B: Biointerfaces. 62: 143–145.

Lee, J.P and B.K. Hwang. 2002. Diversity of antifungal actinomycetes in various vegetatif soils of Korea. Canadian Journal of Microbiology. 48: 407-417.

Lemriss, S., F. Laurent, A. Couble, E. Casoli, J.M. Lancelin, D.S. Bonaccio, S. Rifai, A. Fassaouane and P. Boiron. 2003. Screening of nonpolyenic antifungal metabolites produced by clinical isolates of Actinomycetes. Canadian Journal of Microbiology. 49(11): 669-674.

Lynch, J.M. 1990. The Rhizosphere, John Wiley & Sons Ltd, West Sussex, UK.

Macrae, A., D.L. Rimmer and A.G. O’Donnell. 2000. Novel bacterial diversity recovered from the rhizosphere of oilseed rape (Brassica napus) determined by the analysis of 16S ribosomal DNA. Antonie van Leeuwenhoek. 78: 13–21.

Marschner, P., N. Günter, K. Angelika, W. Laure, and L. Reinhard. 2002. Spatial and temporal dynamics of the microbial community structure in the rhizosphere of cluster roots of white lupin (Lupinus albus L.). Plant Soil 246: 167-174.

Saitou, N. and M. Nei. 1987. The Neighbour-joining method : A New Method for Reconstructing Phylogenetic Trees, Molecular Biology & Evolution 4: 406-426.

Smolander, A. and V. Sundman. 1987. Frankia in acid soils of forests devoid of actinorhizal plants. Physiol Plant. 70: 297-303.

Söderberg, K.H. and E. Bååth. 1998. Bacterial activity along a young barley root measured by the thymidine and leucine incorporation techniques. Soil Biol Biochem. 30: 1259–1268.

Suzuki, S.I., T. Okuda, and S. Komatsubara. 2000. Selective isolation and distribution of Actinobispora strain in soil. Canadian Journal of Microbiology. 46: 708-715.

Thompson, J.D., D.G. Higgins and T.J. Gibson. 1994. Clustal X Version 1.6. User Manual.

Whipps, J. 2001. Microbial interactions and biocontrol in the rhizosphere. J Expr Bot. 52: 487–511.

Downloads

Published

2018-10-20

Issue

Section

Research Articles