Skrining Senyawa Kimia dan Uji Aktivitas Sitotoksik Ekstrak Etanol Teripang Kridou Bintik (Bohadschia argus Jeager) Asal Pantai Harlem Kabupaten Jayapura, Papua

Apianus Kilungga, Linus Y. Chrystomo, Puguh Sujarta

Abstract


The Bohadschia argus Jeager contains bioactive compounds that have the potential as antibiotics, antibacterial, antitumor, anticoagulants and anesthetics and alleviate cancer. This study aims to determine the content of chemical compounds group and to test the cytotoxic activity of ethanol extract of Bohadschia argus Jeager. The method for determining the group of chemical compounds was used the color reaction and precipitation using chemical reagents. The method for testing cytotoxic activity using the Brine Shimp Lethality Test (BSLT), this method commonly was used to test the safety of the use of natural ingredients as traditional medicine. The results of screening of the chemical compound group of  Bohadschia argus Jeager extract showed that the extract contained a lot of alkaloids secondary metabolites and few saponins. The results of testing of cytotoxic activity showed LC50 value of 878.22 ppm. Base on the results of this study it can be concluded that the ethanol extract of Bohadschia argus Jeager has significant cytotoxic activity against Artemia salina Leach, so it can be considered as a chemotherapeutic agent.   

Key words: Screening, chemical compounds, ethanol extract, B. argus, BSLT. 


Full Text:

PDF

References


Andriyanto, E.B., Ardiningsih, P., dan Idiawati, N. 2016. Skrining fitokimia ekstrak daun belimbing hutan (Baccaurea angulata Merr). JKK. 5(4) : 9-13.

Aras, R.S., 2013. Uji toksisitas ekstrak teripang Holothuria Scabra terhadap Artemia Salina. [Skripsi]. Fakultas Ilmu Kelautan dan Perikanan. Universitas Hasanuddin. Makasar.

Arlyza, I.S. 2009, Teripang dan bahan aktifnya, Oseana 34(1): 9-17.

Cahyadi, R. 2009. Uji toksisitas akut ekstrak etanol buah pare (Momordica charantia L.) terhadap larva Artemia salina Leach dengan metode Brine Shrimp Lethality Test (BST). [Skripsi]. Universitas Diponegoro, Semarang.

Doyle, A. dan J.B. Griffiths. 2000. Cell and tissue culture for medical research. John Willey and Sons. New York.

Fransworth, N.R. (1996). Biologycal and phytochemical screening of plants. Journal of Pharmaceutical Science. 55(3): 257-259.

Gerand, R.I., N.H. Greenberg, M.M. MacDonald, A.M.Schumacher and B.J. Abbott. 1992. Protocol for screening chemical agents and natural product against animal tumors and Other Biological System. Cancer Chemother. 3: 59-61.

Harbourne, J.B. 1996. Metode fitokimia penuntun cara modern menganalisis tumbuhan. Edisi kedua. ITB, Bandung.

Kartikasari, S.N., A.J. Marshall, dan B.M. Beehler, (Eds.). (2012). Ekologi Papua. Jakarta. Yayasan Pustaka Obor Indonesia dan Conservation International. Jakarta.

Kustiariyah. 2006. Isolasi, karakterisasi dan uji aktivitas biologis senyawa steroid dari teripang sebagai aprodisiaka alami. [Thesis]. Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Bogor.

Mardany, M.P., L.Y. Chrystomo and A.K. Karim. 2016. Skrining fitokimia dan uji aktivitas sitotoksik dari tumbuhan sarang semut (Myrmecodia beccarii Hook.f) asal Kabupaten Merauke. Jurnal Biologi Papua. 8(1): 13-22.

Meyer, B.N., N.R. Ferigni, J.E. Putnam, L.B. Jaconsen, D.F. Nichols, and J.L. Mclaughlin. 1982. Brine shimp a convenient general bioassay for active plant constituents. Planta Medica. 45: 31-34.

Rita, W.S. 2008. Isolasi dan identifikasi senyawa yang berpotensi sebagai antitumor pada daging buah pare (Momordica charantia L). J. Kimia. 2(2): 6-11.

Robinson, T. 1991. Kandungan organik tumbuhan tinggi (Terjemahan: K. Padmawinata). Institut Teknologi Bandung, Bandung.

Sakarkar, D.M and V.N. Deshmukh. 2011. Ethnopharma-cological review of traditional medicinal plants for anticancer Activity. International Journal of Pharm Tech Research. 3(1) : 298-308.

Sbimizu, Y., E.Inoue and C. Ito. 2004. Effect of tbewator- soluble and non- diatyzable fraction isolated from Sense (Chan Su) on lymphocyte proliferation and natural killer activity in C3H mice. Biol. Phann. Bull. 27(2): 256-269.

Sharma, V., dan R. Paliwal. 2013. Isolation and characterization of saponins from Moringa oleifera (Moringaeceae) Pods. International Journal of Pharmacy and Pharmaceutical Sciences. 5(1) : 179-183.

Simanjuntak, T.L., A.K. Karim, dan L.Y. Chrystomo. 2016. Potensi dan pemanfaatan daun Jilat Yapen (Villebrunea sp.: Urticaceae) secara tradisional oleh masyarakat Kepulauan Yapen dan uji aktivitas toksisitas. Prosiding Seminar Nasional Biologi 2016 Peranan Biologi dalam Peningkatan Konservasi Keanekaragaman Hayati, Fakultas MIPA, Universitas Hasanuddin, Makassar. Hal. 205-214.

Tiwari, A., S. Singh, and S. Singh. 2015. Phytochemical investigation of Caltropis procera flower extract. International Journal of Pharmacy & Life Sciences. 6(2): 4265-4267.




DOI: https://doi.org/10.31957/jbp.633

Refbacks

  • There are currently no refbacks.


View My Stats
CC JURUSAN BIOLOGI FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
UNIVERSITAS CENDERAWASIH
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.