JURNAL BIOLOGI PAPUA https://ejournal.uncen.ac.id/index.php/JBP <p><strong>Jurnal Biologi Papua </strong>JURNAL BIOLOGI PAPUA, ISSN 2086-3314 (print) ISSN 2503-0450 (online) merupakan salah satu jurnal nasional yang diterbitkan oleh Jurusan Biologi FMIPA Universitas Cenderawasih bekerjasama dengan Perhimpunan Biologi Indonesia (PBI) Cabang Papua. Jurnal ini fokus pada ilmu biologi dan serumpun yang terbit setiap Bulan April dan Oktober. JURNAL BIOLOGI PAPUA menerima naskah-naskah terbaik dari penulis yang bersifat asli hasil penelitian maupun telaah (review).</p> Jurusan Biologi FMIPA Universitas Cenderawasih en-US JURNAL BIOLOGI PAPUA 2086-3314 Dampak Pengetahuan, Sikap dan Burnout Terhadap Perilaku Pencegahan Covid-19 Pada Tenaga Kesehatan di Fasilitas Kesehatan Primer Kota Serang https://ejournal.uncen.ac.id/index.php/JBP/article/view/2871 <p>The COVID-19 pandemic has escalated into a global outbreak, with the number of confirmed positive cases reaching hundreds of millions worldwide in March 2023. Its impact extended beyond the healthcare sector, affecting the economy, social culture, and businesses. Healthcare workers at primary healthcare facilities, particularly at Puskesmas, faced a high risk of COVID-19 exposure, necessitating appropriate knowledge, behavior, and attitudes towards virus transmission. Therefore, Puskesmas, as a primary healthcare facility in Indonesia, became the focus of this research to understand and address the impacts of COVID-19 on healthcare workers and the community. Amidst the COVID-19 pandemic, understanding the factors influencing preventive behavior against COVID-19 among healthcare workers was crucial. However, there was a lack of research on the specific factors affecting COVID-19 prevention behavior in Indonesia. Hence, the objective of this study was to investigate the impact of knowledge, attitude, and burnout on COVID-19 prevention behavior among healthcare workers in healthcare facilities in Serang. Partial Least Squares Structural Equation Modeling (PLS-SEM) was utilized to analyze empirical data obtained through simple random sampling. The study collected responses from 181 eligible participants through an online questionnaire. The research findings revealed that the three variables, namely knowledge, attitude, and burnout, had positive and significant impacts on COVID-19 prevention behavior. Among the variables, knowledge had the most substantial direct impact on preventive behavior, followed by attitude and burnout. Notably, burnout negatively influences COVID-19 prevention behavior. This study demonstrated that knowledge, attitude, and burnout significantly influence COVID-19 prevention behavior among healthcare workers, indicating potential applications for healthcare facility management. PLS-SEM model in this research showed moderate predictive accuracy and relevance, suggesting possibilities for further development in future studies. The findings had managerial implications and offered recommendations for future researchers.</p> <p dir="ltr"><strong>Key words</strong>: attitude; awareness; behavior; burnout; preventive.</p> <div> </div> Alexsandro I.S. Lao Constello Di Margt Yohanes Temaluru Copyright (c) 2023 JURNAL BIOLOGI PAPUA 2023-10-01 2023-10-01 15 2 99 110 Pola Sebaran Echinodermata Pada Ekosistem Lamun di Pulau Rhun Maluku Tengah Provinsi Maluku https://ejournal.uncen.ac.id/index.php/JBP/article/view/2717 <p>Echinoderms are benthic fauna whose existence has an impact on the fertility level of the bottom substrate. Aside from being a deposit feeder which destroys most of the sediment, it also causes the process of oxygenation of the top layer of sediment. The purpose of this study was to determine the distribution pattern of echinoderms in the seagrass ecosystem from February to March 2021 on Rhun Island, Central Maluku. This research was carried out on Rhun Island, Banda Naira District. The research location was divided into three stations based on the habitat characteristics at each station. Station I (a beach of fine sand and rocks with a wide expanse of seagrass and a sandy and gravel substrate), Station II (a rocky beach, a fairly wide seagrass with a gravel and sandy substrate). Station III (fine sand beach, seagrass spreaded unevenly and not too dense with gravel and rocky substrate). The results showed that 4 classes of Echinoderms were found with 307 individuals. The physical and chemical environmental factors state that it still met the safe limits for Echinodermata life. The distribution of Echinodermata had a clustered and uniform pattern.</p> <p><strong>Key words</strong>: benthic; Echinodermata; Rhun Island; seagrass.</p> Nur A. Natsir Asyik N. Allifah AF Rosmawati T Zamrin Jamdin Copyright (c) 2023 JURNAL BIOLOGI PAPUA 2023-10-01 2023-10-01 15 2 111 118 10.31957/jbp.2717 Pemanfaatan Ekstrak Kasar Daun Sirih Hijau (Piper betle) dan Daun Sirih Merah (Piper crocatum) Sebagai Antimikroba Pada Daging Ayam Broiler Segar https://ejournal.uncen.ac.id/index.php/JBP/article/view/2881 <p>Green betel plants (<em>Piper betle</em>) and red betel plants (<em>Piper crocatum</em>) are plants that found in many parts of Indonesia which can be categorized as medicinal plants. This is due to the content contained in green and red betel leaves which has phenolic compounds, such as flavonoids, tannins, and saponins. The research objective of this study was to utilize the extract of green betel leaf and red betel leaf by extracting ethanol FG 96% as an antimicrobial compound. There are 4 types of tested bacteria used in this study, they are <em>Escherichia</em> <em>coli</em>, <em>Salmonella</em>, <em>S.</em> <em>aureus</em>, and <em>B. cereus</em>. The extract concentrations used for the two types of leaf extract were 0, 5, 10, 15, and 20%. The sample used for TPC was a fresh broiler chickens, with immersion time for 0, 24, 48, and 72 hours. The total phenolic properties of the green and red betel leaf extracts were 2630.1 mg GAE/g and 6294.8 mg GAE/g, respectively. The total flavonoids possessed by the green and red betel leaf extract were 679.0 mg QE/g and 1662.9 mg QE/g, respectively. Both types of betel leaf extract can function as the best antimicrobial at a concentration of 20%. The bacteria that most resistant to green and red betel leaf extract is <em>E</em><em>.</em><em> coli</em>, and the bacteria that most sensitive to green and red betel leaf extract is <em>S.</em> <em>aureus</em>. The MIC and MBC values of Gram positive bacteria (<em>S.</em> <em>aureus </em>and <em>B.</em> <em>cereus</em>) were lower than the MIC and MBC values of Gram negative bacteria (<em>E</em>.<em> coli</em> and <em>Salmonella</em>).</p> <p><strong>Key words</strong>: Green betel leaves; red betel leaves; extracts; fresh broiler chicken. </p> Adolf J.N. Parhusip Sabrina A. Putridimara Elin Kristianto Alfredo Alfredo Jesseline Fraulencia Copyright (c) 2023 JURNAL BIOLOGI PAPUA 2023-10-01 2023-10-01 15 2 119 129 10.31957/jbp.2881 Bakteri Indigen yang Diisolasi dari Danau Toba: Acinetobacter junii DT2.1 Multiresisten Tembaga, Methylen Blue, dan Basic Fuchine https://ejournal.uncen.ac.id/index.php/JBP/article/view/2804 <p>Lake Toba is one of the industrial waste disposal sites around the location. Copper and dyes are pollutants that are toxic and harmful to organisms. Bioremediation using indigenous bacteria isolated from polluted sites is a promising solution to overcome environmental pollution. Bacteria that have multi-resistance to copper and dyes are expected to increase the efficiency of industrial waste treatment. This study aims to: 1) isolate and characterize copper-resistant bacteria, 2) determine the level of resistance of bacterial isolates to copper, 3) test the resistance of bacteria to dyes. Physiological characterization of bacterial isolates was carried out by observing colony and cell morphology, also Gram staining. Molecular characterization was carried out by analysis of the 16S rDNA gene. The copper resistance level was tested by determining the Minimum Inhibitory Concentration MIC value. The dye resistance test was carried out by growing bacteria on a medium containing 100 ppm of dye. There were eight isolates of copper-resistant bacteria with MIC values ranging from 3-7 mM CuSO<sub>4</sub> with code as DT1.1; DT1.2; DT1.3; DT1.4; DT1.5; DT2.1; DT2.2; and DT3.1. The three highly resistant bacteria were strains DT1.1 and DT1.3 which were identified as <em>Lysinibacillus macroides</em> and strain DT2.1 was identified as <em>Acinetobacter junii</em> with an MIC value of 7mM CuSO<sub>4</sub>, respectively. The results of the resistance test to dyes showed that only <em>A</em><em>.</em><em> junii</em> strain DT2.1 was resistant to 100 ppm Methylene Blue and Basic Fuschine. </p> <p><strong>Key words</strong>: indigenous bacteria; Lake Toba; multi-resistence; dye; copper.</p> Wahyu Irawati Polin P. Ambarita Vannesa El Shaday Sintia Y. Rahmawati Copyright (c) 2023 JURNAL BIOLOGI PAPUA 2023-10-01 2023-10-01 15 2 130 137 10.31957/jbp.2804 Pelestarian Biodiversitas Buah Lokal dan Upaya Pelestariannya oleh Masyarakat di Kabupaten Kampar, Riau https://ejournal.uncen.ac.id/index.php/JBP/article/view/2629 <p>The biodiversity of fruiting plants that grow in various regions in Indonesia is a priceless wealth. Fruiting plants that grow in Indonesia and are planted by Indonesians are called local fruits. This study aimed to identify the types of local fruit that were used by the community and their conservation efforts. The research was conducted in 21 villages in Kampar district. The data was collected through FGDs with local community groups and interviewed with key informants such as village heads, traditional leaders, and community members who owned these local fruit trees. The results showed that there were 12 species of local fruit found in the sample area, namely: Copper Durian (<em>Durio zibethinus </em>L.), Tampui (<em>Baccaurea macrocarpa</em>), Duku (<em>Lansium domesticum </em>Corr.), Mangosteen (<em>Garcinia mangostana </em>L.), Kuwini (<em>Mangifera odorata</em>), Cempedak Hutan (<em>Artocarpus integer</em>), Kapulasan (<em>Nephelium mutabile </em>Blume.), Rambutan (<em>Nephelium lappaceum </em>L.<em>)</em>: (2 varieties, Kapulasan and Rambutan), Guava: Guava (<em>Psidium guajava</em>) and Guava Bol (<em>Syzygium malaccense </em>(L.) Meer): (2 varieties: guava and guava bol), Sawo Manila (<em>Manilkara zapota</em>), Rambai (<em>Baccaurea motyryana </em>Mull.Arg.), Breadfruit (<em>Arthocarpus altilis </em>Fosberg.<em>),</em> Belimbing Wuluh (<em>Averrhoa bilimbi </em>L.) and Ciplukan (<em>Physalis angulate </em>L.). These fruits provided many benefits for the community, not only as a source of vitamins for the family, but also as an additional income for the family economy. Culturally, conservation efforts were carried out by the community replanting and maintaining these plants properly. For the process of plant propagation, the community built nurseries and provided fertilizer for plant nutrition, while the place for planting plants used land such as plantations and in the community's yard. </p> <p><strong>Key words</strong>: fruit biodiversity; local fruits; conservation</p> Nurnatasya Nurnatasya Prima W. Titisari Copyright (c) 2023 JURNAL BIOLOGI PAPUA 2023-10-01 2023-10-01 15 2 138 149 10.31957/jbp.2629 Evaluasi Mutu Fisik dan Penentuan Nilai Sun Protecting Factor (SPF) Sediaan Lotion Ekstrak Etanol Daun Matoa (Pometia pinnata) https://ejournal.uncen.ac.id/index.php/JBP/article/view/2968 <p>Indonesia has high sun exposure, and most people work outdoors, so they need skin protection. One plant with the potential as a sunscreen is matoa leaf (<em>Pometia pinnata</em>). This study aimed to determine the physical quality and the Sun Protecting Factor (SPF) value of matoa leaf ethanol extract lotion, which were analyzed in vitro. Physical quality evaluation included organoleptic tests, homogeneity, pH, adhesion and spreadability, and SPF values obtained by the UV-Vis spectrophotometry method. Mansur Formula was used to calculate the SPF value. The results of the study showed FI (0.5%), FII (1%), and FIII (1.5%) obtained an average SPF value of 13.99 ± 1.45 (maximum protection), 22.27 ± 2.06 (ultra protection), 27.42 ± 0.54 (ultra protection). All formulas had good physical quality and had potential as sunscreens. </p> <p><strong>Key words</strong>: lotion; matoa leaves; SPF; sunscreen</p> Nur F. Bakri Maria J.S Ola Rani D. Pratiwi Copyright (c) 2023 JURNAL BIOLOGI PAPUA 2023-10-01 2023-10-01 15 2 150 158 10.31957/jbp.2968 Hubungan Faktor Risiko dengan Kejadian Malaria di Kampung Nolokla Kabupaten Jayapura https://ejournal.uncen.ac.id/index.php/JBP/article/view/1724 <p>Malaria is a dangerous disease and is still a public health problem in Indonesia, caused by parasites of the genus <em>Plasmodium</em> and transmitted by female mosquitoes of the genus Anopheles. The purpose of this study was to analyze the risk factors for the incidence of malaria and measured the magnitude of the various risk factors that influenced the incidence of malaria in Nolokla Village, East Sentani District, Jayapura Regency. This study used a case control study to find out how far the risk factors included the physical environment, the biological environment and the socio-cultural environment on the incidence of malaria. The case group was all people who declared clinical malaria while the control group was those who were declared free of malaria. The number of samples in this study were 60 respondents, the case samples were taken randomly as many as 30 people and the control group was also 30 people were taken by matching method. The results of the bivariate analysis showed that there were four risk factors, namely the breeding site, the condition of the walls of the house, the presence of shrubs and the presence of <em>Anopheles</em> larvae, and there were six risk factors that had no effect on the incidence of malaria so they were not included in the follow-up test. From the multivariate analysis, it was found that the risk factors that influenced the incidence of malaria were breeding site (<em>p</em>=0.035, OR=3.143, 95%CI 1.066-9.267), condition of house walls (<em>p</em>=0.028, OR=4.500, 95%CI=1.094-18,503), presence of shrubs (<em>p</em>=0.006, OR=5,000, 95%CI=1,510–16,560), presence of <em>Anopheles</em> larvae (<em>p</em> 0.028, OR=3,500, 95%CI=1.112-11.017). The most dominant risk factor that may play a role in the incidence of malaria was the presence of shrubs (Exp=3.095). </p> <pre><strong>Key words</strong>: malaria incidence; API; case control; Nolokla village.</pre> Ivon Ayomi Ign. Joko Suyono Dirk Y.P. Runtuboi Copyright (c) 2023 JURNAL BIOLOGI PAPUA 2023-10-01 2023-10-01 15 2 159 170 10.31957/jbp.1724 Identifikasi Fungi Patogen Penyebab Penyakit Pada Tegakan Pohon Agathis labillardieri di Kampung Rimbajaya Kabupaten Biak Numfor, Papua https://ejournal.uncen.ac.id/index.php/JBP/article/view/3038 <p>This research aimed to determine the presence of disease, characteristics of pathogenic fungi and to identify pathogenic fungi that attack <em>Agathis labillardieri</em> tree stands. This research was conducted on the <em>A. labillardieri</em> plantations in Rimbajaya Village, East Biak District, Biak Numfor Regency and the Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Cenderawasih University from November 2019 to January 2020. The research method began with a field survey, determining samples using purposive sampling, sampling, isolation of pathogenic fungi, purification of isolates, Koch's Postulates, and identification of pathogenic fungi. Based on the research results, it was known that stands of <em>A. labillardieri trees</em> were attacked by leaf rust, stem cancer and conk (fruit body) with isolates from the genus <em>Curvularia</em> and D1N on the leaves, the genus <em>Rhizoctonia</em> on stem cancer and B5M isolates from the conk (fruit body). </p> <p><strong>Key words</strong>: <em>A. labillardieri</em>; fungi; plant disease; pathogen</p> Martha D. Siregar Rosye H.R. Tanjung Copyright (c) 2023 JURNAL BIOLOGI PAPUA 2023-10-01 2023-10-01 15 2 171 184 Pertumbuhan Diameter, Ketebalan dan Bobot Buah Melon (Cucumis melo L.) Akibat Pemberian Dosis Pupuk NPK dan Konsentrasi Pupuk Daun Growmore https://ejournal.uncen.ac.id/index.php/JBP/article/view/2987 <p>Melon has high potential and economic value and has been developed in various fields such as plant breeding, cultivation technology, and the creation of new varieties. Proper cultivation and maintenance techniques are one of the efforts to increase melon crop production through fertilizing with the right type of fertilizer and dosage and concentration. Information on the use of NPK fertilizer and Growmore Fertilizer to increase the diameter, thickness, and weight of melon fruit has not been found. Therefore, this study aimed to increase the diameter, thickness, and weight of melon fruit. This research was expected to get a better diameter, thickness, and weight of melon. This research was conducted in the experimental garden of the Muhammadiyah University of Jember. Research implementation began in January 2023 with an altitude of ± 98 m asl. This study used a randomized block design (RBD) with two factors and three replications, namely: NPK fertilizer: P0 = 0 g/plant (control), P1 = 600 kg/ha (15 g/plant), P2 = 800 kg/ha (20 g /plant). Growmore leaf ertilizer N0 = 0 g/l (control), N1 = 3 g/l, N2 = 4 g/l (125 ml/plant). Observational variables consisted of: planted fruit weight (kg), fruit weight per plot (kg), fruit diameter (mm), and flesh thickness (mm). In the variables of planting fruit weight and fruit weight per treatment plot that gave the highest yields were P1 with yields of 1.14 kg and 7.52 kg, N2 with yields of 1.09 kg and 6.68 kg, and P1N2 interactions gave the highest yields of 1 .28 kg and 8.20 kg. The variable diameter of the treated fruit that gave the highest yield was P1 with a yield of (24.30 mm) N2 with a yield of (23.09 mm and the P1N2 interaction gave the highest yield of 127.89 mm. On the variable thickness of the treated fruit, the highest yield was P2 with a yield of (5.47 mm, N2 with a yield of (4.61 mm), and P1N2 interaction gave the highest yield of 38.00 mm. </p> <p><strong>Key words</strong>: diameter; thickness; melon fruit; NPK; growmore </p> Bagus Tripama Abdul Jalil Ferdian A. Wahyudi Andri Wahyudi Pio T. Ananda Copyright (c) 2023 JURNAL BIOLOGI PAPUA 2023-10-01 2023-10-01 15 2 185 192 10.31957/jbp.2987 Keunikan Struktur Anatomi dan Morfologi Burung Maleo (Macrochepalon maleo) sebagai Fauna Endemik Indonesia dan Upaya Konservasinya https://ejournal.uncen.ac.id/index.php/JBP/article/view/2980 <p>Maleo bird is one of the unique animals of the aves class which is included in endemic animals in Indonesia. The Maleo bird is almost endangered due to predators and irresponsible behavior of local people. One of the conservation efforts carried out by the government is contained in Law No. 5 of 1990 and Government Regulation No. 7 of 1999 concerning the protection or conservation of Maleo birds. The purpose of this paper is to analyze the morphological and physiological characteristics of the Maleo bird (Macrochepalon maleo), the anatomy of the Maleo bird (Macrochepalon maleo), the relationship of the uniqueness of the behavior of the Maleo bird (Macrochepalon maleo) <em> with the morphology, anatomy, and physiology of the Maleo bird, as well as conservation efforts of the Maleo bird (</em>Macrochepalon<em> </em>maleo). The method used is a literature review by reading various books, journals and papers and other publications in accordance with the research topic. The results of research that have been conducted show that the morphology, anatomy, and physiology of the Maleo bird are closely related to the behavior of the Maleo bird. The Maleo bird uses a crest located on its head to detect heat when digging an egg nest. Maleo bird legs equipped with sharp hooves can make Maleo birds able to survive in coastal forests. Maleo birds are monogamous animals, namely animals that are loyal to their partners. The advice that can be given through this literature review is that the community must have awareness to take care of the Maleo bird as a form of accountability for the mandate given by God to humans.</p> <p class="JRPMBody"><strong>Key words</strong>: Aves; endemic; conservation; maleo; morphology</p> Damai Y. Manalu Elisabeth M.T. Rido Wahyu Irawati Copyright (c) 2023 JURNAL BIOLOGI PAPUA 2023-10-01 2023-10-01 15 2 192 203 10.31957/jbp.2980