ANALYSIS OF ANTIOXIDANT ACTIVITY OF LIDAH MERTUA LEAVES (Sansevieria trifasciatalauretii) USING DPPH METHOD
DOI:
https://doi.org/10.31957/jipi.v10i2.2195Keywords:
antioxidant, mother-in-law's tongue leaf, total phenol content, total flavonoid content.Abstract
Lidah mertua plant  has the potential as a natural antioxidant and the antioxidant activity of a plant depends on the number of active compounds extracted. This study aims to determine the yield, total phenol content, total flavonoid content, and IC50 value of lidahmertua leaf extract macerated with ethanol, ethyl acetate, and n-hexane as solvents, and to determine the compounds contained in the lidahmertua leaf extract which have activity antioxidants. The results of the phytochemical test of the lidahmertua leaf extract showed positive results containing polyphenolic compounds, flavonoids, saponins, alkaloids, and steroids. The maceration yield of each solvent was 12.13%, 10.15% and 8.11%, respectively. While the measurement results of the total phenol content of each solvent were 6.96; 3.67; and 1.94 mg GAE/g. The results of the measurement of the flavonoid content of each solvent were 5.82; 3.28; and 1.71 mg QE/g. The IC50 values for each solvent were 162.135 mg/L, 184.561 mg/L and 206.265 mg/L. The extract which has the highest total phenol content, total flavonoid content, and antioxidant activity is in ethanol solvent.
Downloads
References
Almey, A., Khan, A.J., Zahir S., Suleiman M., and Aisyah Rahim K., 2010, Total phenolic content and primary antioxidant activity of methanolic and ethanolic extract of aromatic plants’leaves, International Food Research, 17 :1077-1088;
Alothman M, Bhat R, Karim AA. UV irradiation-induced changes of antioxidant capacity of fresh-cut tropical fruits. Innov Food Sci Emerg Technol. 2009; 10: 512-6;
Andayani, R., Y. Lisawati, dan Maimunah, 2008, Penentuan aktivitas, kadar fenolat total dan likopen pada buah tomat (Solanum lycopersicum L). Jurnal Sains dan teknologi farmasi, 13: 1 – 9;
Basma A. A., Zakaria., Lactha Y. L., &Sasidharan S., 2011, Antioxidant activity and Phytochemical Screening of The Methanol Extracts of Euphorbia hirta L, Asian Pasific Journal of Tropical Medicine, 15(2011): 386-390;
Bogoriani, N. W., A. A. I. M. Laksmiwati , A. A. B. Putra, W. E. Heltyanim K. D. P. Lestari, P. A. E. Mahayani, 2019, Saponins Role of Bali Andong Leaf As Antiobesity In Rats, International Journal of Pharmaceutical Research, 11(2): 383 – 389;
Chang C, Yang M, Wen H, Chern J (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Analaysis, 10: 178-182;
Hanani, E., 2005, Identifikasi Senyawa Antioksidan dalam Sponscallispongia Sp. Dari Kepulauan Seribu, Majalah Ilmu Kefarmasian, 2 (3): 127-133;
Harborne, J.B., 1987, Metode Fitokimia. Penentuan cara modern menganalisis tumbuhan, ITB, Bandung;
Hernani dan M. Raharjo, 2005, Tanaman Berkhasiat Antioksidan, Jakarta: Penebar Swadya. Widjaya, A. 1996. Radikal Bebas dan Parameter Status Antioksidan. Forum Diagnosticum. 4.(1996): 1-6;
Ionita, P., 2005, Is DPPH Stable Free Radical a Good Scavenger for Oxygen Active Species, Chem. Pap., 59 (1): 11-16;
Illing, I., Safitri, W. & Erfiana, E. (2017). Uji Fitokimia Ekstrak Buah Dengen. Journal of Mathematics and Natural Science, 8(1), 66-84;
Langseth, L., 1995, Oxidant, Antioxidant, and Desease Prevention, International Life Science Institute press, Belgium.
Marliani, L., Kusriani, H., dan Indah N.S., (2014). Aktivitas Antioksidan Daun dan Buah Jambang (Syzigium Cumini L) Skeel, Prosiding SnaPP, Sains, Teknologi dan Kesehatan, ISSN 2089-3584,EISSN 2303-2480213;
Markham, K.R. and Bloor, S.J. (1998). Analysis and identifikasi of flavonoids in practice. In, Flavonoids in health and disease ( C.A. Rice-Evans and L,, Packer, eds), Marcel Dekker
Molyneux, P., (2004), The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol 26- (2): 211:219;
Ningdyah, A. W, Alimuddin, A.H, Jayuska, A., 2015, Uji Toksisitas dengan Metode BSLT (Brine Shrip Lethality Test) terhadap hasil fraksinasi ekstrak kulit buah Tampoi (Baccaurea marcocarpa), Journal Kimia Khatulistiwa, 4(1);
Prakash, A., Rigelhof, F., Miller, E., 2001, Medallion Laboratories: Analytical Progress, Antioxidant Activity, www.terranostrachocolate.com /files/ Comparative_and_General_Antioxidant_Information.pdf, diunduh pada 20 Juli 2019;
Pratama, Rahadian, Departemen Biokimia, Fakultas Matematika, D. A. N. Ilmu, and Pengetahuan Alam. 2010. “Potensi Antioksidan Dan Toksisitas Ekstrak Daun Sansevieria Cylindrica.â€Skripsi. Institut Pertanian Bogor.FMIPA;
Robinson, T. (1995). Kandungan Organik Tumbuhan Tingkat Tinggi. Bandung, ITB Press;
Rohman, A. dan Riyanto, S., 2005, Aktivitas Antioksidan Ekstrak Buah Mengkudu (Morinda Citrifolia L,), J. Agritech, 25 (3): 131-136;
Rohman, A., Riyanto, S., dan Utari, D., 2006, Antioxidant activities, total phenolic and flavonoid contents of ethyl acetate extract of Mengkudu (Morinda citrifolia, L) fruit and its fractions, Majalah Farmasi Indonesia 17, 136-142;
ShanmugapriyaR, Ramathan T, Thirunavu p., 2011, Evalution of antioxidant potential and antibacterial activity of Acalypha indica Linn. Using In vitro model, Asian Journal of Biomedical and pharmaceutical Sciences;
Waters Corporation, 2005, MassLynx 4.1: Getting Started Guide, Waters Corporation, Washington D.C;
Whika Febria Dewatisari, Leni Rumiyanti, Ismi Rakhmawati, Jl Soekarno, and Hatta No. n.d. “Rendemen Dan Skrining Fitokimia Pada Ekstrak Daun Sanseviera Sp . Rendemen and Phytochemical Screening Using Leaf Extract Of.†17(3):197–202.
Winarsi, H., 2007, Antioksidan Alami dan Radikal Bebas, Kanisius, Yogyakarta;
Yoshihiro, C., Hiroshi, O., Mayumi, O. K., Tadahiro, N., &Tojiro, T. 1996. Structural Identification of Two Antioxidant quinic acid derivatives from garland (Chrysanthemum coronarium L.). J. Agric. Food Chem, 44: 2037-2039.
