Pertumbuhan dan Perkembangan Protocorn Like Bodies (PLB) Anggrek Papua Dendrobium discolor dengan Perlakuan Auksin dan Sitokinin

https://doi.org/10.31957/jbp.1299

Authors

  • Agustriani Warpur Mahasiswa PS Magister Biologi Universitas Cenderawasih
  • Linus Y. Chrystomo Universitas Cenderawasih
  • Suharno Suharno Universitas Cenderawasih

Abstract

Dendrobium could meet the demands of domestic and foreign consumers from time to time because of their very charming flower blossoms. Orchid flowers are attractive because of the variety their color, size, shape, composition, number of buds the blossom of flowers, especially for the color it is vary greatly. One of the potential Dendrobium Papua orchids is Dendrobium discolor. To fulfill the needs of Dendrobium orchids in pots or in peaces it is depend on the success of Protocorn Like Bodies (PLB) cultivation. The purpose of this study was to determine the growth and development of PLB cultivated in vitro on Vacint & Went (V&W) base media with a combination of different plant growth regulators. The method used wasPLB cultivation with tissue culture techniques in vitro on V&W media with a factorial completely randomized design (CRD). The first factor was auxin treatment (2,4D) with 5 concentrations 1, 2, 3, and 4 ppm plus control (without plant growth regulator treatment), while the second factor was cytokinin treatment with 5 concentrations 1, 2, 3, and 4 ppm plus 1 control (without plant growth regulator treatment). Each treatment repeated 5 times, threfore, there are 125 treatment units in total 125 treatment units. The media used were Vacin and Went (VW). The variables measured were leaf length and the number of leaves per plantlet in each combination treatment of plant growth regulator (PGR) after one month of cultivation. Data were analyzed by using variance (ANOVA) to determine the significance. The results of this study indicated that the combination treatment of auxin and cytokinin PGR in V&W media had a significant effect on the length and number of plantlets leaves and the most significant treatment was the combination of PGR auxin: cytokinin = 0:3 ppm. So it can be concluded that the best medium for PLB culture is a combination of PGR auxin: cytokinin = 0:3 ppm. with an average value of 3.5 cm plantlet leaf length and number of 6 leaves per plantlet. 

Key words: Protocorn Like Bodies; tissue culture; V&W medium; auxins; cytokinins.

Downloads

Download data is not yet available.

Author Biographies

Agustriani Warpur, Mahasiswa PS Magister Biologi Universitas Cenderawasih

Kultur Jaringan Tumbuhan

Linus Y. Chrystomo, Universitas Cenderawasih

Biology

Suharno Suharno, Universitas Cenderawasih

Biology

References

Abidin, Z. 1994. Dasar-dasar pengetahuan tentang zat pengatur tumbuh. Penerbit Angkasa Bandung.

Agustini, V., Suharyanto, Suharno, L. Dimara, and C.D. Sembay. 2013. The diversity of tropical orchids of South Papua. Jurnal Biologi Papua. 5(1): 1–9.

Amilah, dan Y. Astuti. 2006. Pengaruh konsentrasi ekstrak taoge dan kacang hijau pada media Vacin & Went (VW) terhadap pertumbuhan kecambah anggrek bulan (Phalaeonopsis amabilis L.). Buletin Penelitian No. 9 Tahun 2006.

Andalasari. T.D., Yafisham, dan Nuraini. 2014. Respon pertumbuhan anggrek Dendrobium terhadap jenis media tanam dan pupuk daun. Fakultas Pertanian Universitas Bandar Lampung Jurnal Penelitian Pertanian Terapan. 14(3): 167-173.

Aqil, M., dan R. Efendi. 2015. Aplikasi SPSS dan SAS untuk perancangan percobaan, aplikasi pertanian, peternakan, kehutanan dan MIPA. Penerbit Absolute Media, Yogyakarta. 192 hal.

Fandani, H.S., S.N. Mallomasang, dan I.N. Korja. 2018. Keanekaragaman jenis anggrek pada beberapa penangkaran di Desa Ampera dan Desa Karunia Kecamatan Palolo, Kabupaten Sigi. Jurnal Warta Rimba. 6(3): 9-14.

Firoozabady, E., and Y. Moy. 2004. Regeneration of pineapple plants via somatic embryogenesis and organogenesis. J. In Vitro Cell Dev Biol. Plant. 40: 67-74.

Fonnesbech, M. 1992. Growth hormone and propagation of Cymbidium in vitro. Physiol. Plant. 27(3): 310-321.

Hartanto, A., A. Haris, dan D.S. Widodo. 2009. Pengaruh kalsium, hormon auksin, giberellin dan sitokinin terhadap pertumbuhan dan perkembangan tanaman jagung. Jurnal Kimia Sains dan Aplikasi. 12(3): 72-75.

Kuntoro, B., A.J.F. Wiliam, dan A. Tumbuan. 2015. Potensi pengembangan agribisnis bunga anggrek di Kota Batu, Jawa Timur. BPTP. Jawa Timur.

Kuswandi, P.C. 2012. Menumbuhkan semangat berwira usaha dengan memanfaatkan bioteknologi melalui pengenalan aklimatisasi anggrek hasil kultur jaringan. Makalah PPM.

Martin, K.F., and J. Madassary. 2006. Rapid in vitro propagation of Dendrobium hybrid through direct shoot formation from foliar explant and protocorn like bodies. J. Sci.Hortic. 108: 95-99.

Reddy, D., and R. Dharaneeswara. 2014. Effects of 6-Benzyl amino purine (6-Bap) on in vitro shoot multiplication of grand naine (Musa sp.). International Joernal of Advanced Bioteknology and Research. 5(1): 36-42.

Saad, I., M. Abobkar, and A.M. Elshahed. 2012. Plant tissue culture media. Intech. Departemen of Botani and Microbiology, Faculty of Science, Sebha University, Libya.

Sahrawat, A.K., and S. Chand. 2001. Continuous somatic embryogenesis and plant regeneration of hypocotyls segments of Psoralea corylifolia Lin. An endangered and medicinally important fabaceae plant. Current Science. 81(10): 1328-1331.

Salisbury, B.F., and C.W. Ross. 1995. Fisiologi tumbuhan. Terjemahan. oleh Diah R. Lukman, dan Sumaryono. Penerbit ITB Bandung.

Sinar Tani, 2001. Penyuluhan pertanian. Yayasan Pengembang-an Sinar Tani, Jakarta.

Sripaoraya, S., R. Marchant, J.B. Power, and M.R. Davey. 2003. Plant regeneration via somatic embryogenesis and organogenesis of commercial pineapple (Ananas comosus L.). J. In Vitro Cell Dev. Biol. Plant. 39: 450-454.

Thomas, T.D., and R. Chaturvedi. 2008. Endosperm culture: A novel method for triploid plant. J. Tiss. Org. Cult. 93(1): 1–14.

Widiastoety, D. 2014. Pengaruh auksin dan sitokinin terhadap pertumbuhan planlet anggrek Mokara. J. Hort. 24(3): 230–238.

Yusnita. 2003. Kultur jaringan cara memperbanyak tanaman secara efisien. Agro Media Pustaka. Jakarta.

Downloads

Published

2021-04-01

Issue

Vol. 13 No. 1 (2021)

Section

Research Articles

License

1. Copyright Policy

Jurnal Biologi Papua is committed to promoting the dissemination, accessibility, and responsible reuse of scholarly research in biology and related disciplines.

Under this policy, authors retain copyright ownership of their published articles and grant Jurnal Biologi Papua the non-exclusive right of first publication.

Authors retain the right to use, distribute, reproduce, and share their scholarly work in accordance with the applicable publication license.

The journal acknowledges the intellectual contributions of authors and respects their moral rights, including the right to be properly identified as creators of their work.

2. Publication License

Articles published under this policy are distributed under the Creative Commons Attribution 4.0 International License (CC BY 4.0).

License URL: https://creativecommons.org/licenses/by/4.0/

Under this license, users are permitted to:

  1. Share: Copy and redistribute published articles in any medium or format.

  2. Adapt: Remix, transform, and build upon published materials for any purpose, including commercial purposes.

  3. Redistribute: Make articles available through repositories, websites, databases, and other scholarly platforms.

These permissions are subject to the Creative Commons Attribution 4.0 International License.

Users must provide appropriate credit to the original authors, include a link to the license, and indicate whether changes have been made.

Reuse must not imply endorsement by the authors or publisher.

3. Rights of Authors

Authors publishing in Jurnal Biologi Papua retain the following rights:

  1. Copyright ownership of their original scholarly work.

  2. The right to distribute and share their published articles.

  3. The right to deposit their articles in institutional or subject repositories.

  4. The right to reuse their work in subsequent scholarly publications, subject to appropriate acknowledgment and applicable ethical standards.

  5. The right to share their work for teaching, research, academic collaboration, and other lawful purposes.

Authors must acknowledge the original publication in Jurnal Biologi Papua when distributing or reusing the published version.

4. Rights of the Publisher

Authors grant Jurnal Biologi Papua the non-exclusive right to publish, disseminate, archive, and preserve accepted manuscripts.

The journal may reproduce and distribute the published version through its official website and authorized indexing, archiving, and scholarly dissemination services.

The publisher is responsible for maintaining the integrity of published content and accurately displaying copyright and licensing information.

5. Open Access Policy

Jurnal Biologi Papua supports immediate open access to scholarly research.

Articles published under this policy are freely accessible to readers without subscription requirements or access fees.

The journal encourages the responsible dissemination and reuse of published research to support scientific progress, academic collaboration, and the broader exchange of knowledge.

Published content may be accessed, downloaded, shared, and reused in accordance with the applicable Creative Commons license.

6. Third-Party Materials

Authors are responsible for ensuring that all materials included in their manuscripts comply with applicable copyright and intellectual property laws.

Permission must be obtained when required for the use of third-party figures, photographs, tables, illustrations, or other copyrighted materials.

Materials excluded from the article's Creative Commons license must be clearly identified with their applicable copyright and permission notices.

The journal's publication license does not override the rights of third-party copyright holders.

7. Repository and Self-Archiving Policy

Authors are permitted and encouraged to deposit and share their published articles through institutional repositories, academic repositories, and personal or institutional websites.

Authors must provide appropriate acknowledgment of the article's original publication in Jurnal Biologi Papua, including its citation information and DOI where available.

Repository deposits and subsequent distribution must comply with the license applicable to the relevant version of the article.

8. Licensing Information and Article Metadata

The applicable copyright holder and publication license should be clearly identified on each published article's webpage.

Where supported, licensing information should also be included in article metadata and the published PDF.

The journal is committed to maintaining accurate and consistent licensing information across its online publishing platform and scholarly indexing services.

9. Policy Application and Previously Published Articles

This policy applies to manuscripts accepted following its formal adoption by the journal and publisher.

Articles published before the adoption of this policy remain subject to the copyright arrangements and licenses applicable at the time of their publication, unless a valid change is authorized by the relevant rights holders.

The journal will not apply a new license retroactively without the necessary authorization.

10. Policy Commitment

Jurnal Biologi Papua is committed to transparent copyright arrangements, responsible open access publishing, and respect for intellectual property rights.

The journal will periodically review its copyright and licensing practices to maintain consistency with recognized standards of scholarly communication.

References

Creative Commons Attribution 4.0 International License: https://creativecommons.org/licenses/by/4.0/

Directory of Open Access Journals (DOAJ): https://doaj.org/apply/copyright-and-licensing/