Uji Efek Analgetik Infusa Daun Beluntas (Pluchea indica L.) Pada Mencit Jantan Galur Swiss
DOI:
https://doi.org/10.31957/jbp.436Abstract
Beluntas leaves (Pluchea indica L.) have been known as analgetic reducer. The study about this research has been going on right now. The goal of this research is to study the analgesic effect of beluntas (P. indica) leaf infused into male mice of swiss strain. The method used is the stretching chemical stimuli using acetic acid as an inducer of pain. Healthy male mice of Swiss strain were divided into five groups, and each group consisted of 5 mice. Group I was given paracetamol at a dose of 65 mg/kg of body weight, group II were given distilled water , the group III-V were given beluntas leaf infuse in the variation of 10 %, 20 % and 40 %. Thirty minutes after test substance application, acetic acid of 100 mg/kg of body weight were given intraperitoneally in all groups and stretching of mice was observed every 5 minutes for 1 hour. The data was analyzed by using normality test of Kolmogorov-Smirnov; the  test of homogeneity of variance was analyzed by ANOVA and post hoc tests were to differenciate the percentage analgetic of every group. Statistical test showed normal distributed and homogeneous data; there are significant differences of percentage analgesic between paracetamol and beluntas leaf infuse of 10, 20, and 40 % (p < 0.05). There was significant differences between infuse of beluntas leaves with positive control (parasetamol) in mice.Â
Key words: Analgetic, leave of beluntas (P. indica), infuse, mice.
Downloads
References
Afrianti, R., R. Yenti dan D. Meustika. 2014. Uji aktivitas analgetik ekstrak etanol daun papaya (Carica papaya L.) pada mencit putih jantan yang diinduksi Asam Asetat 1%. Jurnal Sains Farmasi & Klinis. 1(1): 54–60.
Badan Penelitian Pengembangan Kesehatan (Balitbangkes). 2001. Inventaris tanaman obat Indonesia (I). Jilid 2. Depar-temen Kesehatan Republik Indonesia. Jakarta.
Gunawan, S.G. 2007. Farmakologi dan terapi. edisi 5. Depar-teman Farmakologi dan Terapeutik Fakultas Kedokteran Universitas Indonesia. Jakarta.
Hasti, S., N.H. Sandi, dan T. Srianti. 2012. Uji efek antipiretik ekstrak etanol, fraksi n-heksana dan fraksi etil asetat daun beringin (Ficus benjamina L.) pada tikus putih jantan (Rattus norvegicus). Jurnal Sains dan Teknologi. 17(1): 40-43.
Hastuti, S. dan I.A Safitri. 2015. Aktivitas analgetika ekstrak etanol daun sligi (Phyllanthus buxifolius Meull.Arg) terhadap mencit galur balb/c. Indonesian Journal on Medical Science. 2(1): 11–15.
Iranloye, B.O., V.B. Owoyele, O.R. Kelani, dan S.B. Olaleye. 2011. Analgesic activity of aquoeus leaf extract of Phyllanthus amarus. Afr. J. Med. Sci. 40(1): 47–50.
Mali, A.A., D.D. Bandawane, dan M.G. Hivrale. 2013. Anti-inflammatory and analgesic activities of ethyl acetate and petroleum ether fractions of Cassia auriculata Linn. Leave. Research Article. 13: 191–197.
Noridayu, A.R., Y.F. Yii, A. Faridah, S. Khozirah, and N. Lajis. 2011. Antioxidant and antiacetylcholinesterase activities of Pluchea indica Less. International Food Research Journal. 18(3): 925–929.
Nurhalimah, H., N. Wijayanti, dan T.D. Widyaningsih. 2015. Efek antidiare ekstrak daun beluntas (Pluchea indica L) terhadap mencit jantan yang diinduksi bakteri Salmonella typhimurium. Jurnal Pangan dan Agroindustri. 3(3): 1083–1094.
Parmar, N.S., dan S. Prakash. 2006. Screening methods in pharmacology. Apha Science International. Oxford.
Puspitasari, S., S. Listyawati dan T. Widiyani. 2003. Ativitas analgetik ekstrak umbi teki (Cyperus rotundus L.) pada mencit putih (Mus musculus L.) jantan. Biofarmasi 1(2): 50–57.
Sibarani, V.R., M.W. Pemsi. dan A. Henoch. 2013. Uji analgetika ekstrak daun beluntas (Pluchea indica L) pada mencit (Mus musculus). Jurnal e-Biomedik. 1(1): 621–628.
Sirait. 2007. Penuntun fitokimia dalam farmasi. ITB. Bandung.
Solihatifati, S.F., dan A. Widodo. 2010. Pengaruh ekstrak rimpang temu kunci (Kaempferia pandurata Roxb.) terhadap jumlah geliat mencit Balb/C yang diinduksi Asam Asetat. Artikel Ilmiah. Universitas Diponegoro Semarang
Sugiyono. 2010. Metode penelitian pendidikan. Penerbit Alfa-Beta. Bandung.
Sukaryana, Y. dan Y. Priabudiman. 2014. Pengaruh pemberi-an ekstrak daun beluntas (Pluchea indica L) terhadap total kolesterol darah Broiler. Jurnal Penelitian Pertanian Terapan. 14(3): 152-157.
Tamsuri, A. 2007. Konsep dan penatalaksanaan nyeri. Penerbit Buku Kedokteran EGC, Jakarta.
Tjay, T.H., dan K. Rahardja. 2002. Obat-obat penting. PT. Elex Media Komputindo. Jakarta.
Wulandari, D., dan P. Hendra. 2011. Efek analgesik infusa daun Macaranga tanarius L. pada mencit betina galur Swiss. Jurnal Ilmu-ilmu Hayati dan Fisik. 13(2): 108-116.
