Pertumbuhan dan Perkembangan Otak Fetus Mencit Setelah Induksi Ochratoxin A Selama Periode Organogenesis

https://doi.org/10.31957/jbp.518

Authors

  • Arum Setiawan Jurusan Biologi Fakultas MIPA, Universitas Sriwijaya, Palembang
  • Mammed Sagi Laboratorium Embriologi dan Histologi Hewan, Fakultas Biologi Universitas Gadjah Mada, Yogyakarta
  • Widya Asmara Laboratorium Mikrobiologi, Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta
  • Istriyati Istriyati Laboratorium Embriologi dan Histologi Hewan, Fakultas Biologi Universitas Gadjah Mada, Yogyakarta

Abstract

This experiment was performed to examine the effects of Ochratoxin A (OTA) during organogenesis period on fetuses brain growth and development. Thirty pregnant mice were divided randomly into 5 groups of 6. Ochratoxin A was dissolved in sodium bicarbonate and administered orally on seventh to fourteenth days of gestation at dosage of 0.5, 1.0, 1.5 mg/kg bw, respectively. The remaining animals were used as  an untreated control, and placebo were given by  Sodium Bicarbonate. At the age of 18-day pregnancy, mice were sacrified dan taken its brains. The fetuses brain growth and development  were observed by measure brain weight, cereberum width and length, cerebellum width and length, and the wall thichkness of cerebrum. Result of this studies indicated that OTA caused decreased of brain weight, the length and width of cerebrum and cerebellum, the wall thickness of cerebrum significantly.

Key words: Ochratoxin A, brain, cerebrum, cerebellum, mice. 

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Author Biographies

Arum Setiawan, Jurusan Biologi Fakultas MIPA, Universitas Sriwijaya, Palembang

Jurusan Biologi Fakultas MIPA, Universitas Sriwijaya, Palembang

Mammed Sagi, Laboratorium Embriologi dan Histologi Hewan, Fakultas Biologi Universitas Gadjah Mada, Yogyakarta

Laboratorium Embriologi dan Histologi Hewan, Fakultas Biologi Universitas Gadjah Mada, Yogyakarta

Widya Asmara, Laboratorium Mikrobiologi, Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta

Laboratorium Mikrobiologi, Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta

Istriyati Istriyati, Laboratorium Embriologi dan Histologi Hewan, Fakultas Biologi Universitas Gadjah Mada, Yogyakarta

Laboratorium Embriologi dan Histologi Hewan, Fakultas Biologi Universitas Gadjah Mada, Yogyakarta. 

References

Bangalore, L. 2007. Brain development. Chelsea House Publisher. USA. p: 110.

Belmadani A., G. Tramu, A.M. Betbeder and E.E. Creppy. 1998. Subchronic effects of ochratoxin A on young adult rat brain and partial prevention by aspartame, a sweetener. Hum. Exp. Toxicol. 17: 380–386.

Darmanto W., E. Prihiyantoro dan R. Harmonis. 2004. Induksi 2-Methoxyethanol pada masa prenatal sebagai penyebab kelainan otak pada mencit. Ber. Pen. Hay., 10: 1–5.

Gondona J.M., M.P. Martin and M. Cifuentes. 1996. Epyndemal denudation, aqueductal obliteration and hydrochepalus after a single injection of neuroaminidase into the lateral ventricle of adults rats. J. Neurophat. Exp. Neurol. 55: 999-1008.

Marti N.B. 2006. Ochratoxin A and ochratoxigenic modulds in grapes, must and wine, ecophysiological study, tesis doctoral Universitat de Lleida Spain, from URL: http://www.tesisenxarxa.net/TESIS_UdL/AVAILABLE/TDX0406107172700/Tbmn10de18.pdf. 10 Juni 2007.

Miraglia M. and C. Brera. 2002. Assessment of Dietary Intake of Ochratoxin A by the Population of EU Member States. Directorate General Health and Consumer Product, Rome, Italy.

Naruse I. and Y. Tsutsui. 1989. Brain abnormalities induced by murine cytomegalovirus injected into the cerebral ventricles of mouse embryos exo utero. Teratol. 40: 181–189.

Ohta, K., M. Maekawa, R. Katagiri, E. Ueta and I. Naruse. 2006. Genetic susceptibility in the neural tube defects induced by Ochratoxin A in the genetic archiencephaly mouse, Pdn/Pdn. Congen. Anom. Kyo. 46:144-148.

Purvess D., G.J. Augustine, D. Fitzpatrick, L.C. Katz, A.S. Lamantia, J.O. McNamara and S.M. William. 2001. Neuroscience 2nd ed., Sinauer Associates Inc. Massachusetts. 409–426.

Sava V., O. Reunova, A. Velasques, R. Harbison and J. Sanchez-Ramos. 2006. Acute neurotoxic effects of the fungal metabolite Ochratoxin A. Neuro. Toxicol. 27:82-92.

Setiawan, A., M. Sagi, W. Asmara dan Istriyati. 2011. Analisis kuantitatif sel purkinje cerebellum mencit (Mus musculus L.) Swiss Webster setelah induksi ochratoxin A selama periode organogenesis. J. Biota. 16(2): 262–268.

Setiawan, A., M. Sagi, W. Asmara dan Istriyati. 2012. Analisis pertumbuhan kartilago epifisialis Os Tibia fetus mencit (Mus musculus L.) Swiss Webster setelah induksi Ochratoxin A selama periode organogenesis. J. Biol. Papua 4(1): 25–31.

Smith J.N., dan S. Mangkoewidojo. 1988. Pemeliharaan, pembiakan dan penggunaan hewan percobaan di daerah tropis. UI press. Jakarta. p: 356.

Taylor P. 1986. Practical teratology. Academic Press Inc. London. p: 265.

Theiler K., 1989, The house mouse : atlas of embryonic development. Springer-Verlag, New York. pp: 26–53.

Ueta, E., M. Kodama, Y. Sumino, M. Kurome, K. Ohta, R. Katagiri and I. Naruse. 2009. Gender-dependent differences in the incidence of ochratoxin A-induced neural tube defects in Pdn/Pdn mouse, Con.Anom. Manuscript ID:CGA-08-2009-043.R2.

Wilson J.G. 1973. Environment and birth defects. Academic Press, New York. pp: 92–94.

Zhang X., C. Boesch-Saadatmandi, Y. Lou, S. Wolfram, P. Huebbe and G. Rimbach. 2009. Ochratoxin A induces apoptosis in neuronal cells. Genes. Nutr. 4: 41–48.

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Published

2018-10-13

Issue

Vol. 5 No. 1 (2013)

Section

Research Articles

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