Keragaman Bakteri Enterik Patogen Siput Felle (Angulyagra tricostata) dari Pesisir Danau Sentani Kabupaten Jayapura

https://doi.org/10.31957/jbp.522

Authors

  • Dais Iswanto Bagian Biologi Kedokteran, Fakultas Kedokteran Universitas Cenderawasih
  • Daniel Lantang Staf Pengajar Pascasarjana Biologi, FMIPA, Universitas Cenderawasih, Jayapura
  • Tri Gunaedi Staf Pengajar Pascasarjana Biologi, FMIPA, Universitas Cenderawasih, Jayapura

Abstract

Felle Snails (Angulyagra tricostata) is an alternative source of animal protein for the people around Sentani Lake. Meat of Felle snails has an indication of being contaminated by pathogenic enteric bacteria harmful to the health of the public consumption. The purpose of this study was to determine the diversity of bacterial enteric pathogens in Felle snails (A. tricostata) from the coast of Sentani Lake, Jayapura. Felle snails were taken from various points near existing residential areas around the coastal region of Sentani Lake. Extraction of meats Snail dilutions was made prior to planting in stages up to 10-10. Each serial dilution of 0.1 mL were taken and inoculated into Lactose Broth medium (LB), Mac Conkey Media (MC) agar and media EMBA at 370C for 24 hours. Colonies that grew were sub-cultured to get different colonies and grown on agar slant. Identification of individual characters was performed by colony fenetik numerical methods. The result indicated that there were seven selected isolates and the dendrogram showed that those species were similar to four of genus Enterobacteriaceae. Seven isolates consisted of 2 isolates similar to the genus Escherchiae, 2 isolates similar to Klebsiellae, 2 isolates similar to the genus Salmonellae and 1 isolate similar to the genus Shigellae. Similarity index value of each  selected isolates ranged from 69 to 86%.

Key words: Felle snail, bacterial enteric pathogens, Enterobacteriaceae, and sentani lake.

Downloads

Download data is not yet available.

Author Biographies

Dais Iswanto, Bagian Biologi Kedokteran, Fakultas Kedokteran Universitas Cenderawasih

Bagian Biologi Kedokteran, Fakultas Kedokteran Universitas Cenderawasih

Daniel Lantang, Staf Pengajar Pascasarjana Biologi, FMIPA, Universitas Cenderawasih, Jayapura

Staf Pengajar Pascasarjana Biologi, FMIPA, Universitas Cenderawasih, Jayapura

Tri Gunaedi, Staf Pengajar Pascasarjana Biologi, FMIPA, Universitas Cenderawasih, Jayapura

Staf Pengajar Pascasarjana Biologi, FMIPA, Universitas Cenderawasih, Jayapura

References

Adagbada, O. 2011. Profile bacteriology meat snails of different sites. Nigerian Institute of medical Research, Yaba. Lagos State, Nigeria.

Beaudoin, R N., R.D. Danielle, D.L. Durkee and S.E. Barbaroo. 2007. Isolation of bacteriophage from sewage sludge and characterization of its bacterial host cell. Rivier Academy Journal. 3(1): 1–8.

Brener, D.J., N.R. Krieg and J.T. Staley. 1992. Bergeys manual of systematic bacteriology. Second edition. Vol. Two, Part B. Springer, USA.

Efuntoye, M.O., O.O. Mabekoje, and F.A. Adekoya. 2011. Biochemical enterotoxigenity and antibiogram profiles Staphylococcus aureus isolated from snails. Journal Microbiology Nigeria Institute. 3: 47–50.

Ekundayo, E,O., and S.O. Fagade. 2006. Microbial flora associated with the soils of edible snail farm in Southern Nigeria. Nigerian Journal of Soil Science. 15(1): 75–80.

Enji, C.A., A.U. Ogogo, C.A. Emmanuel-Ikpeme and O.E.D. Okon. 2008. Nutritional assesment of some Nigerian land and water snails species. Ethiopian Journal of environmental studies and management. 1(2): 56–60.

Onyuka, J.H.O., R. Kakai, D.M. Onyango and P.F. Arama. 2011. Prevalence and antimicrobial susceptibility patterns of enteric bacteria isolated from water and fish in Lake Victoria basin Western Kenya. International Journal of Biology Medical science. 1(1): 6–13.

Priest, F and B. Austin. 1993. Modern bacterial taxonomy. Second Edition. Champman dan Hall. London.

Radji, M., A. Puspaningrum dan A. Sumiati. 2010. Deteksi cepat bakteri Escehirchia coli dalam sampel air dengan metode polymerase chain reaction menggunaan primer 16E1 dan 16E2. Makara Sains. 14(1): 39–43.

Reeves, W.L., R.T. Dillon, Jr. and G.A. Dasch 2008. Freshwater Snails from Common Wealth of Dominica, The America Mollucas. Bulletin. 24: 59-63.

Said, N.I dan R. Marsidi. 2005. Mikroorganisme patogen dan parasit di dalam air limbah domestik serta alternatif teknologi pengelolaannya. JAI. 1(1): 65–81.

Sudrajat, D., M. Lina R., dan F. Suhadi. 2000. Deteksi cepat bakteri Escherichia coli enterohemoragik (EHEK) dengan metode Polymerase Chain Reaction. Risalah Pertemuan Ilmiah Penelitian dan Pengembangan Teknologi Isotop dan Radiasi. Tahun 2000.

Surbakti, S. Br., A. Basukriadi dan M.P. Patria. 2008. Studi biologi dan ekologi Thiaridae (moluska: gastropoda) di danau Sentani, Papua serta kelestariannya. Seminar Nasional Sains dan Teknologi II. Universitas Lampung. 17–18 November 2008. hal: 608–619.

Waluko, F.A., D. Djokosetiyanto, Kholil dan D. Soedarma. 2008. Analisis strategi dan pengelolaan dan peran lembaga dalam rangka konservasi danau Sentani Jayapura. Jurnal Media Konservasi. 13: 21–31.

Yenny dan E. Herwana. 2007. Resistensi dan bakteri enterik: Aspek global terhadap antimikroba. Universa Medica. 26(1): 46–56.

Yvonne, H., Y. Agokei, and K. George. 2000. Occurence enteric bacteria of edible snails, Niger Delta Nigeria. Departement of Food Science and Technology. University of Uyo, Nigeria.

Downloads

Published

2018-10-14

Issue

Vol. 5 No. 2 (2013)

Section

Research Articles

License

1. Copyright Policy

Jurnal Biologi Papua is committed to promoting the dissemination, accessibility, and responsible reuse of scholarly research in biology and related disciplines.

Under this policy, authors retain copyright ownership of their published articles and grant Jurnal Biologi Papua the non-exclusive right of first publication.

Authors retain the right to use, distribute, reproduce, and share their scholarly work in accordance with the applicable publication license.

The journal acknowledges the intellectual contributions of authors and respects their moral rights, including the right to be properly identified as creators of their work.

2. Publication License

Articles published under this policy are distributed under the Creative Commons Attribution 4.0 International License (CC BY 4.0).

License URL: https://creativecommons.org/licenses/by/4.0/

Under this license, users are permitted to:

  1. Share: Copy and redistribute published articles in any medium or format.

  2. Adapt: Remix, transform, and build upon published materials for any purpose, including commercial purposes.

  3. Redistribute: Make articles available through repositories, websites, databases, and other scholarly platforms.

These permissions are subject to the Creative Commons Attribution 4.0 International License.

Users must provide appropriate credit to the original authors, include a link to the license, and indicate whether changes have been made.

Reuse must not imply endorsement by the authors or publisher.

3. Rights of Authors

Authors publishing in Jurnal Biologi Papua retain the following rights:

  1. Copyright ownership of their original scholarly work.

  2. The right to distribute and share their published articles.

  3. The right to deposit their articles in institutional or subject repositories.

  4. The right to reuse their work in subsequent scholarly publications, subject to appropriate acknowledgment and applicable ethical standards.

  5. The right to share their work for teaching, research, academic collaboration, and other lawful purposes.

Authors must acknowledge the original publication in Jurnal Biologi Papua when distributing or reusing the published version.

4. Rights of the Publisher

Authors grant Jurnal Biologi Papua the non-exclusive right to publish, disseminate, archive, and preserve accepted manuscripts.

The journal may reproduce and distribute the published version through its official website and authorized indexing, archiving, and scholarly dissemination services.

The publisher is responsible for maintaining the integrity of published content and accurately displaying copyright and licensing information.

5. Open Access Policy

Jurnal Biologi Papua supports immediate open access to scholarly research.

Articles published under this policy are freely accessible to readers without subscription requirements or access fees.

The journal encourages the responsible dissemination and reuse of published research to support scientific progress, academic collaboration, and the broader exchange of knowledge.

Published content may be accessed, downloaded, shared, and reused in accordance with the applicable Creative Commons license.

6. Third-Party Materials

Authors are responsible for ensuring that all materials included in their manuscripts comply with applicable copyright and intellectual property laws.

Permission must be obtained when required for the use of third-party figures, photographs, tables, illustrations, or other copyrighted materials.

Materials excluded from the article's Creative Commons license must be clearly identified with their applicable copyright and permission notices.

The journal's publication license does not override the rights of third-party copyright holders.

7. Repository and Self-Archiving Policy

Authors are permitted and encouraged to deposit and share their published articles through institutional repositories, academic repositories, and personal or institutional websites.

Authors must provide appropriate acknowledgment of the article's original publication in Jurnal Biologi Papua, including its citation information and DOI where available.

Repository deposits and subsequent distribution must comply with the license applicable to the relevant version of the article.

8. Licensing Information and Article Metadata

The applicable copyright holder and publication license should be clearly identified on each published article's webpage.

Where supported, licensing information should also be included in article metadata and the published PDF.

The journal is committed to maintaining accurate and consistent licensing information across its online publishing platform and scholarly indexing services.

9. Policy Application and Previously Published Articles

This policy applies to manuscripts accepted following its formal adoption by the journal and publisher.

Articles published before the adoption of this policy remain subject to the copyright arrangements and licenses applicable at the time of their publication, unless a valid change is authorized by the relevant rights holders.

The journal will not apply a new license retroactively without the necessary authorization.

10. Policy Commitment

Jurnal Biologi Papua is committed to transparent copyright arrangements, responsible open access publishing, and respect for intellectual property rights.

The journal will periodically review its copyright and licensing practices to maintain consistency with recognized standards of scholarly communication.

References

Creative Commons Attribution 4.0 International License: https://creativecommons.org/licenses/by/4.0/

Directory of Open Access Journals (DOAJ): https://doaj.org/apply/copyright-and-licensing/