Efek Sitotoksik Ekstrak Daun Ketapang (Terminalia catappa L.) Pada Sel Kanker Payudara T47D

Barinta Widaryanti, Nur Khikmah, Nunung Sulistyani

Abstract


Cancer is the second cause of death in the world after heart disease. Cancer therapy using natural product was considered has no side effect. Secondary metabolites from ketapang (Terminalia cattapa L) leaves has a prospect for cancer therapy. The objective of this research was to determine the cytotoxical effect of ketapang leaves on T47D breast cancer cells. Ketapang leaves were macerated using chloroform, ethyl acetate and methanol. Cytotoxicity effect were determined using MTT Cell Viability Assay. The result showed that chloroform, ethyl acetate and methanol extract were not able to reduce cell viability of T47D cells. 

Key words: cancer, ketapang, T. cattapa, cytotoxic, T47D cells.


Full Text:

PDF

References


Chu, S.C., S.F. Yang, S.J. Liu, W.H. Kuo, Y.Z. Chang, and Y.S. Hsieh. 2007. In vitro and in vivo antimetastatic effect of Terminalia catappa L. leaves on lung cancer cells. Food and Chemical Toxicology. 45: 1194-1201.

Da’i, M., A. Fiveri, dan E. Meiyanto. 2007. Efek sitotoksik ekstrak tanaman keladi tikus (Typhonium divaricatum (L.) terhadap sel HeLa. Jurnal Farmasi Indonesia. 3(4): 163–167.

Ellington, A.A., M. Berhow, and K.W. Singletary. 2005. Induction of macroautophagy in human colon cancer cells by B-group soybean saponins. Carcinogenesis. 26: 159-167.

Ferguson, P.J., E. Kurowska, D.J. Freeman, and D.J. Koropatnick. 2004. A flavonoid fraction from cranberry extract inhibits proliferation of human tumor cell lines. J. Nutr. 134: 1529-1535.

Kampa, M., V. Alexaki, G. Notas, A. Nifli, A. Nistikaki, A. Hatzoglou, Bakogeorge, Efstathia, E. Koumtzoglou, G. Blekas, D. Boskou, A. Gravanis, and E. Castanas. 2004. Antiproliferatif and apoptotic effect of selective phenolic acids on T47D human breast cancer cells: Potential mechanisms of action. Breast Cancer Res. 6: 63-74.

Mathivadani, P., P. Shanthi, and P. Sachdanandam. 2007. Apoptotic effect of Semecarpus anacardium nut extract on T47D cancer cell line. Cell Biology International 31: 1198-1206.

Muhammad, A. and S.Y. Mudi. 2011. Phytochemical screening and antimicrobial activities of Terminalia catappa leaves extracts. Biochemistri. 23(1): 35-39.

Parton, M., M. Dowsett, and I. Smith. 2001. Studies of apoptosisis in breast cancer. BMJ. 322: 1528-1532.

Pebriana, R.B., B.W.K. Wardhani, E. Widayanti, N.L.S. Wijayanti, T.R. Wijayanti, S. Riyanto dan E. Meiyanto. 2008. Pengaruh ekstrak metanolik daun kenikir (Cosmos caudatus Kunth.) terhadap pemacuan apoptosis sel kanker payudara. Pharmacon. 9(1): 21–26.

Putra, A., Tjahjono, dan Winarto. 2012. Efektivitas ekstrak umbi Typhonium flagelliforme fraksi diklorometanolik dalam menghambat proliferasi sel MCF-7 kanker payudara. J Indon Med Assoc. 62(1): 10-15.

Rahmawati, E., Sukardiman, dan A.F. Muti. 2013. Aktivitas antikanker ekstrak n-heksana dan ekstrak metanol herba pacar air (Impatiens balsamina Linn) terhadap sel kanker payudara T47D. Media Farmasi. 10(2): 47-55.

Ruth, S.M. 2005. Environmental influence on isoflavone and saponins in soybeans and their role in colon cancer. Journal of Nutrition. 135: 1239-1242.

Siswandono. 1983. Mekanisme kerja obat-obat antikanker. Buletin ISFI Jatim Tahun X. 3: 1-2.

Sugiyanto, B. Sudarto, E. Meiyanto, A.E. Nugroho, dan U.A. Jennie. 2003. Aktivitas antikarsinogenik senyawa yang berasal dari tumbuhan. Majalah Farmasi Indonesia. 14: 206-225.

Susilowati, S., E. Meiyanto, Sugiyanto. 2012. EFEK ekstrak etanolik daun Gynura procumbens (Lour) Merr terhadap proliferasi sel kanker payudara tikus yang diinduksi 7,12- Dimetilbenz(a)antrasen (DMBA) http://dosen. narotama.ac.id/wp-content/uploads/2012/02. Download, 1 September 2016.

Venkatalakshmi, P., P. Brinda, and K. Induja. 2014. In vitro anti oxidant and anti tumor studies on Terminalia catappa Bark. International of Pharmacy and Pharamceutical Sciences. 6: Supll 1.

Witantri, R.G. E.C.A. Ruspendi, D.S. Saputro. 2015. Keanekaragaman pohon berpotensi obat antikanker di kawasan Kampus Kentingan Universitas Sebelas Maret, Surakarta, Jawa Tengah. Pros Sem Nas Masy Biodiv Indon. 1(3): 477-483.




DOI: https://doi.org/10.31957/jbp.54

Refbacks

  • There are currently no refbacks.