Fotodegradasi, Uji pH dan Kandungan In Vivo Pigmen Klorofil Lamun Thalasia hemprichii
DOI:
https://doi.org/10.31957/acr.v1i2.932Abstract
Pigmen klorofil memiliki peran penting dan besar terhadap berbagai sistem kehidupan di perairan laut, dan dalam aplikasinya telah banyak digunakan untuk berbagai produk makanan, minuman, farmasi, dan kosmetik, sehingga studi terkait stabilitasnya perlu dilakukan. Tujuan penelitian ini adalah mengetahui kandungan, pengaruh sinar, dan pengaruh pH terhadap pigmen klorofil Thalasia hemprichii. Metode penelitian yang digunakan adalah (1) pengukuran kandungan secara in vivo, (2) ekstraksi aseton, (3) iradiasi, dan (4) uji pH. Hasil penelitian menunjukkan bahwa purata kandungan klorofil Thalasia hemprichii pada kedalaman 1 m = 15,42 spad/cm2, kedalaman 2 m = 17,82 spad/cm2, dan kedalaman 3 m = 19,01 spad/cm2. Pengaruh sinar merah selama 7 jam iradiasi menyebabkan degradasi pigmen di daerah Qx = 26,77% dan pada daerah Qy = 29,05% per jam, sedangkan iradiasi cahaya polikromatik selama 7 jam menyebabkan degradasi pada daerah Qx = 52,81% dan pada daerah Qy = 54,80%. Hasil uji pH menunjukkan bahwa klorofil lamun Thalasia hemprichii bersifat stabil pada pH 7 (netral), mengalami degradasi pada pH bersifat asam dan basa. Konsentrasi pH bersifat basa memiliki serapan tinggi, yaitu pH 9 (Qx = 0,732798 dan Qy = 0,362952), pH 11 (Qx = 0,879036 dan Qy = 0,433446), dan pH 13 (Qx = 0,948732 dan Qy = 0,459035), sedangkan pH bersifat asam memiliki serapan yang kecil, yaitu pH 5 (Qx = 0,787641 dan Qy = 0,391379) dan pH 1 (Qx = 0,736968 dan Qy = 0,264192).
Kata Kunci:Â Fotodegradasi; pH; Kandungan in vivo;Â Klorofil;Â Thalasia hemprichiiÂ
Downloads
References
Anonim, 2005. Menguak Misteri Lamun. http://duamata.blogspot.com/2005/12/menguak-misteri-lamun.html. Akses 14 Juli 2008.
Anonim, 2006. Lamun Berpotensi Sebagai Makanan Kesehatan. http://images.google.co.id/imgres?imgurl=http://biogen.litbang.deptan.go.id/berita_artikel/images/tabel_lamun.Png&imgrefurl=http//biogen.litbang.deptan.go.id/berita_artikel/serbi_2006_lamun.Php&h=159&w=421&sz=5&hl=id&start=7&tbnid=EbhgYc_rRC_6vM:&tbnh=47&tbnw=125&prev=/images%3Fq%3Djenisjenis%2Blamun%2Bdi%2Bindonesia%26gbv%3D2%26hl%3Did%26sa%3DG. Akses 14 Juli 2008.
Bittencourt, C., Marcella, P., Felicissimo, Jean-Jacques Pireaux, and Houssiau. 2005. Study of Annatto from Bixa orellana Seeds: An Application of Time-of-flight Secondary Ion Mass Spectrometry. Article Spectroscopy Europe, Vol. 17 No. 2 (2005). www.spectroscopyeurope.com. Akses 14 Juli 2008.
Dutton, H.J., Bayley, G.F., and Kohake, E., 1943. Dehydrated spinach changes in color and pigments during processingand storage. Ind. Eng. Chem., 35, 1173-1177.
Fennema, O.R., 1996. Food Chemistry: 3rd ed. New York: Marcel Dekker, Inc.
Gross, J., 1991. Pigment in Vegetables; Chlorophyll and Carotenoids. New York: Van Nostrand Reinhold.
Heriyanto, dan Limantara, L. 2006. Kandungan Klorofil in Vivo Daun Cincau Hitam, Cincau Perdu, Cincau Hijau, dan Cincau Minyak. Fakultas Biologi Universitas Kristen Satya Wacana. Salatiga.
Kimbal, J.W. 1992. Biologi Jilid I. Jakarta: Erlangga.
Koca, N., Karadeniz, F., and Burdurlu, H.S. 2003. Effect of pH on Chlorophyll Degradation and Colour Loss in Blanched Green Peas. Faculty of Engineering, Food Engineering Departement, Ankara University. Turkey.
Lagowski, J.J. 1997. Macmillan Encyclopedia of Chemistry. New York: Simon & Schuster and Prentice Hall International.
Laleh, G.H., Frydoonfar, H., Heidary, R., Jemeei, R., and Zare, S. 2006. The effeect of light, temperature, pH, and spesies on stability of Anthocyanin pigments in four berberis species. Pakistan Journal of Nutrition, 5(1), 90-92.
Limantara, L., Koehler, P., Wilhelm, B., Porra, R.J. and Scheer, H. 2006. Photostability of bacteriochlorophyll-a and derivatives: potential sensitizer for photodinamic tumor theraphy. Photochemistry and Photobiology, 82, 770-780.
Montenegro, M.A., A. De O. Rios, A.Z. Mercadante, M. Nazareno, A., and Borsarelli, C.D. 2004. Model studies on the photosensitized isomerization of bixin. Journal of Agricultural and Food Chemistry, 52, 367-373.
Nurcahyanti A.D.R. Limantara, L. 2007. Fotodegradasi Ekstrak Kasar, Klorofil a, dan Fucoxanthin Padina australis dan Dictyota crenulata. Prosiding Seminar Nasional Pigmen 2007 ‘Back to Nature dengan Pigmen Alami’. Universitas Kristen Satya Wacana. Salatiga.
Purwati, A. 2006. Lamun Berpotensi Sebagai Sumber Makanan Kesehatan. http://www.indobiogen.or.id/berita_artikel/serbi_2006_lamun.php. Akses 14 Juli 2008.
Rahayu, P., dan Limantara, L. 2005. Studi lapangan kandungan klorofil in vivo beberapa spesies tumbuhan hijau di Salatiga dan sekitarnya. Seminar Nasional MIPA 2005, Universitas Indonesia.
Rahayu, P., Notosoedarmo, S., dan Limatara, L. 2006. Studi Lapangan Kandungan Klorofil in Vivo Daun Katuk dan Katuk Badak Di Salatiga dan Tawangmangu. Fakultas Biologi Universitas Kristen Satya Wacana. Salatiga.
Sajilata, dan Singhal. 2006. Isolation and stabilisation of natural pigments for food application. Stewart Postharvest Review, 5, 11.
Scotter, M.J., Thorpe, S.A., Reynolds, S.L., Wilson, L., and Strutt, A. 1994. Characterizations of the principal colouring components of annatto using high performance liquid chromatography with photodiode-array detection. Food Additives and Contaminants, 11(3), 301-315.
Scotter, M.J., Wilson, L.A., Appleton, G.P., and Castle, L. 1998. Analysis of annatto (Bixa orellana) food coloring formulations. 1. determination of colouring components and colored thermal degradation products by high performance liquid chromatography with photodiode-array detection. Journal of Agricultural and Food Chemistry, (46), 1031-1038.
Silva, G.S., Souza, A.G., Botelho, J.R., Silva, M.C.D., Silva, T.M.S. 2007. Kinetics study of norbixin’s first stage thermal decomposition, using dynamic method. Journal of Thermal Analysis and Calorimetry, 87(3), 871–874.
Suparmi, Limantara, L., dan Prasetyo, B. 2008. Karakterisasi stabilitas pigmen norbixin dari biji kesumba (Bixa orellana L.). Prosiding Seminar Nasional 2008 ‘Sains dan Teknologi Pigmen’, Universitas Kristen Satya Wacana, Salatiga.
Suparmi, Prasetyo, B., dan Limantara, L. 2007. Fotodegradasi Pigmen Bixin Dari Biji Kesumba (Bixa orellana L.): Potensinya Sebagai Pewarna Alami Makanan. Prosiding Seminar Nasional Pigmen 2007 ‘Back to Nature dengan Pigmen Alami’, UKSW Salatiga, B (05):195-204.
Tomascik, T., Mah, A.J., Nontji, A., and Moosa, M.K. 1997. The Ecology of Indonesian Seas: Part Two. Periplus edition.
Wiles, D.M., and Carlsson, D.J. 1987. Mc Graw-Hill Encyclopedia of Science & Technology. New York: Mc Graw-Hill Book Company 13(6): 337-379.
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously
licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License
that allows others to share the work with an acknowledgement of the work's authorship and initial
publication in this journal. - Authors are able to enter into separate, additional contractual arrangements for the non-exclusive
distribution of the journal's published version of the work (e.g., post it to an institutional repository
or publish it in a book), with an acknowledgement of its initial publication in this journal. - Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or
on their website) prior to and during the submission process, as it can lead to productive exchanges,
as well as earlier and greater citation of published work (See The Effect of Open Access).
