Analisis Kromosom Varietas Kacang Gude (Cajanus cajan (L.) Millspaugh) Asal Pulau Kisar, Maluku Barat Daya (MBD)
DOI:
https://doi.org/10.31957/jbp.1138Abstract
Pigeon pea (Cajanus cajan) is one of the biodiversity of Kisar Island, Maluku Barat Daya (MBD). This study aimed to determine the number and size of chromosomes from pigeon pea (C. cajan) from Kisar Island, MBD. The method used for chromosome observation is the squashing method, while for the analysis of the shape and size of chromosomes using the picsart and image raster applications. Data analysis using descriptive analysis with the help of figures and tables. The results showed that the chromosome number of three varieties of pigeon pea (C. cajan) was 2n = 14. The longest chromosome is owned by orange seed varieties that are 9.17 µm, batik color seed varieties are 9.09 μm, the shortest chromosomes are owned by black seed varieties of 8.32 µm. The chromosome length of the three varieties of pigeon pea (C. cajan) varies showing different genome numbers.  Â
Key words: Chromosome; Cajanus cajan; Kisar Island; Maluku Barat Daya.Â
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Aiyeloja, A.A., and O.A. Bello. 2006. Ethnobotanical potentials of common herbs in Nigeria: A case study of Enugu state. Educational Review. 1(1): 16-22.
Anjali, M., and A.K. Srivastava. 2012. Karyological studies in twelve accessions of Carthamus tinctoriusi. Caryologia. 65(1): 1-6.
Bennet, P.N, and D.R. Laurence. 1987. Clinical Pharmacology. Sixth Edition. Churchill Livingstone, Edinburgh.
Buitendijtk, J.H., E.J. Noon, dan M.S. Ramanna,. 1997. Nuclear DNA content in twelve species of Alstroemeria L. and some of their hybrids. Annals of Botany. 79(4): 343-353.
Cook, B.G., B.C. Pengelly, S.D. Brown, J.L. Donnelly, D.A. Eagles, M.A. Franco, J. Hanson, B.F. Mullen, I.J. Partridge, M. Peters, and R. Schultze-Kraft. 2005. Tropical forages: an interactive selection tool. Cajanus cajan. CSIRO, DPI & F (Qld), CIAT, and ILRI, Brisbane, Australia.
de Sousa, A.C.B., R. Godoy, D.A. Sforça, T. de Campos, M.I. Zucchi, L. Jank, and A.P. de Souza. 2011. Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers. Scientia Agricola. 68(4): 431-439.
Dewitte, A., L. Leus, T. Eeckhaut, I. Vanstechelman, J. Van Huylenbroeck, and E. Van Bockstaele. 2009. Genome size variation in Begonia. Genome. 52(10): 829–838.
Emefiene, M.E., A.B. Salaudeen, and A.Y. Yaroson. 2013. The use of pigeon pea (Cajanus cajan) for drought mitigation in Nigeria. International Letters of Natural Sciences. 2(12): 6-16.
Escudero, M., S. Martin-Bravo, I. Mayrose, M. Fernandez-Mazuecos, and O. Fiz-Palacios. 2014. Karyotypic Changes through dysploidy persist longer over evolutionary time than polyploid changes. PLoS One. 9(1): 1-7.
Grant, V. 1958. The regulation of recombination in plants. Cold Spring Harbor Symposia on Quantitative Biology. 23: 337–363.
Greilhuber, J., and R. Obermayer. 1998. Genome size variation in Cajanus Cajan (Fabaceae): A reconsideration. Plant Systematics and Evolution. 212(1): 135-141.
Imai, H. T., Y. Satta, M. Wada, and N. Takahata. 2002. Estimation of the highest chromosome number of eukaryotes based on the minimum interaction theory. Journal of Theoritical Biology. 217(1): 61–74.
Iswantari, W., T. Mulyaningsih, dan A. Muspiah. 2017. Karyomorfologi dan jumlah kromosom empat grup Gyrinops versteegii (Gilg.) Domke. di Lombok. Jurnal Ilmu Kehutanan. 11(2): 205-211.
Saxena, K.B., R.K. Saxena, and R.V. Kumar. 2012. Evidence of a unique inter-allelic epistatic interaction for seed coat color in pigeonpea (Cajanus cajan L.) Millspaugh. Euphytica. 186: 813–816.
Klamt, A., and M.T. Schifino-Wittmann. 2000. Karyotipe morphology and evolution in some Lathyrus (Fabaceae) species of Southern Brazil. Genetics and Molecular Biology. 23(2): 46-7.
Kumar, C.V.S., S.J. S. Naik, N. Mohan, R.K. Saxena, and R.K. Varshney. 2007. Botanical Description of Pigeonpea [Cajanus Cajan (L.) Millsp.]. In: The Pigeonpea Genome. Compendium of Plant Genomes book series (CPG). Springer, pp. 17-29.
Martin E., H. Akan, M. Ekici, and Z. Aytac. 2011. Karmophological studies on section bucerates boiss of Trigonella L. (Leguminosae) from Turkey. Caryologia. 61(3): 225-236.
Ogbunugafor, H.A., M.N. Igwo-Ezikpe, I.O. Igwilo, T. Salisu, and C.N. Ezekwesili. 2013. Cajanus cajan: Potentials as Functional Food. Dept. of Applied Biochemistry, Nnamdi Azikiwe University, Awka. Nigeria.
Ohri, D. 1998. Genome size variation and plant systematics. Annals of Botany. 82 (Supplement A): 75-83.
Patil, H.E., N. Dikshit, S.A. Aklade, and P.A. Vavdiya. 2016. Evaluation of vegetable type pigeon pea (Cajanus cajan (L.) genotypes for yield and yield contributing traits. Ecology, Environment and Conservation. 22: 247-254.
Peruzzi, L., G. Goralski, A.J. Joachimiak, and G. Bedini. 2012. Does actually mean chromosome number increase with latitude in vascular plants? An answer from the comparison of Italian, Slovak and Polish floras. Compative Cytogenetics. 6(4): 371–377.
Kolnicki, R.L. 2000. Kinetochore reproduction in animal evolution: Cell biological explanation of karyotypic fission theory. Proceedings of the National Academy of Sciences. 97(17): 9493–9497.
Qin, Z., X. Li, D. Liu, Q. Wang, L. Lu, and Z. Zhang. 2019. Analysis of chromosome karyotype and genome size in echiuran Urechis unicinctus Drasche, 1880 (Polychaeta, Urechidae). Comparative Cytogenetics. 13(1): 75–85.
RodrÃguez, J.M. 2015. Analysis of the germination rate mitotic index and karyotype of Chromolena barranquillensis (Hieron.). Universidad del Norte, Colombia.
Setyawan, dan Sutikno. 2000. Karyotipe kromosom pada Allium sativum (Bawang Putih) dan Pisum sativum (Kacang Kapri). Jurnal BioSmart. 2(1): 14-20.
Sharma, S., N. Agarwal, and P. Verma. 2011. Pigeon pea (Cajanus cajan L.): A hidden treasure of regime nutrition. Journal of Functional and Environmental Botany. 1(2): 91–101.
She, C.W., and X.H. Jiang. 2015. Karyotype analysis of Lablab purpureus (L.) sweet using fluorochrome banding and fluorescence in situ hybridisation with rDNA probes. Czech Journal of Genetics and Plant Breeding. 51(3): 110–116.
Sing, N.P., A. Das, S.K. Chaturvedi, A. Bohra, and D. Datta. 2015. Biology of Cajanus cajan (pigeon pea). Ministry of Environment, Forest and Climate Change: Government of India.
Kar, S., and B.K. Datta. 2017. Floral biology of Cajanus cajan (L.) Millsp. [Leguminosae] in Tripura (India). Pleione. 11(1): 104-115.
Sarsamkar, S.S., S.B. Borgaonkar, S.V. Kalyankar, B.P. Kadam, and G.R. Kadam. 2008. Genetic variability studies in pigeon pea [Cajanus cajan (L.) Millsp.]. International Journal of Plant Sciences. 3(2): 502-503.
Stebbins, G.L. 1996. Chromosomal evolution in higher plants. Edward Arnold, London.
Suryo. 2003. Genetika manusia. Universitas Gadjah Mada Press. Yogyakarta.
Udensi, O., E.A. Edu, V.O. Ntuil, P.A. Otu, I.S. Urual, and G.A. Akpan. 2011. Cyto-sensitivity of some locally grown pigeon pea [Cajanus cajan (L.) Millsp] to amiprophos methyl treatment as a guide for improvement. International Journal of Current Research. 3(11): 176-180.
van der Maesen, L.J.G. 1990. Pigeonpea: origin, history, evolution and taxonomy. In: The Pigeonpea (Nene YL, Hall SD, Sheila VK eds.), pp 15-46, CAB International.
Varshney, R.K., W. Chen, and Y. Li. 2012. Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers. Nature Biotechnology. 30(1): 83–89.
Young, H.A., G. Sarath, and C.M. Tobias. 2012. Karyotipe variation is indicative of subgenomic and ecotipic differentiation in switchgrass. BMC Plant Biology. 12(1): 117-127.
Zayed, E.M., and A.E. Zeinab. 2016. Karyotype Analysis and Protein Profile for Three Trifolium Species. Journal of Cytology & Histology. 7(2): 1-6.
