Phytochemical Screening, Antibacterial and Cytotoxic Activity of Dendobium lasianthera J.J.Sm. from Papua
DOI:
https://doi.org/10.31957/jbp.1289Abstract
The aims of the study are to evaluate bioactive compounds, antibacterial and cytotoxic potential of D. lasianthera. This orchid grows well all over New Guinea Island as an ornamental plant because of their beautiful flowers. Orchids also known rich of its phytochemical compounds which already used as a traditional medicine in many countries around the world. However, research in pharmacological fields is still limited. In this study, leaves and stem of D. lasianthera were powdered and extracted with ethanol followed by fractionated using n-hexane, ethyl acetate, and ethanol solvent. Extract as well as fraction were tested for phytochemical screening and determined antibacterial activity against Escherichia coli and Staphylococcus aureus using Disc Diffusion Method. Brine Shrimp Lethality Test (BSLT) was used to observe cytotoxic potential of leaves and stem extract and fraction at 10, 50, 100, 250, 500, 750, and 1000 ppm. The results showed that the leaves and stem extract contained tannin and alkaloids, separately. The ethanol extract of D. lasianthera showed 7.35 mm (leaves) and 7.43 mm (stem) inhibition zone against S. aureus Furthermore, the maximum inhibition zone of ethanol fraction of leaves and n-hexane fraction of stem were 8.42 mm and 8.10 mm separately. The LC50 of stem extract and fractions in these study were 699.3 ppm (ethanol extract), 602.1 ppm (ethyl acetate), 329.6 ppm (n-hexane fraction) and 676 ppm (ethanol fraction), whereas for leaves, only ethyl acetate fraction has toxict activity with an average LC50 833.2 ppm.Â
Key words: D. lasianthera; phytochemical screening; antibacterial; cytotoxicity; Papua.Â
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References
Agustini, V., Suharyanto, Suharno, L. Dimara, and C.D. Sembay. 2013. The diversity of tropical orchids of South Papua. Jurnal Biologi Papua. 5(1): 1–9.
Agustini, V., S. Sufaati, dan Suharno. 2015. Anggrek dataran tinggi Habema di Distrik Nanggo, Trikora, Jayawijaya Papua. Jurnal Biologi Papua. 7(1): 9-15.
Behera, D., D.C.C. Rath,, and U. Mohapatra. 2013. Medicinal Orchids in India and their conservation : A Review. Floriculture and Ornamental Biotechnology 7(1):53-59.
Besra,R.C.,L.Majhee and J. Sharma. 2011. Evaluation of phytochemical,antioxidant and hepatoprotective activitu of tuber of Geodorum laxiflorum Griff. Journal of Pharmacology and Toxicology 6(7):610-623
Bulpitt, C.J., Y. Li., P.F. Bulpitt, and J. Wang. 2007. The use of orchids in Chinese medicine. Journal of the Royal Society of Medicine. 100: 558-563.
Cahyo, F. A and D Dinarti. 2015. pengaruh iradiasi sinar gamma terhadap pertumbuhan protocorm like bodies anggrek D. lasianthera (JJ. Smith) secara in vitro. J. Hort Indonesia. Vol 6(3): 177-186
Davis,WW and Stout,TR.1971. Disc plate method of microbiological antibiotic assay : factors influencing variability and error 1. Appl Microbiol:22 ($):659-665
Devi, P.U., S. Selvi, D. Devipriya, S. Murugan, and S. Suja. 2009. Antitumor and antimicrobial activities and inhibition of in vitro lipid peroxidation by Dendrobium nobile. African Journal of Biotechnology. 8 (10) : 2289-2293.
Ganapaty, S., M. Ramaiah, K. Yasaswini, V. K. Nuthakki, D. Harikrishnareddy. 2013. Quantitative phytochemical estimation and evaluation of hepatoproytective activity of methanolic extract of Dendrobium ovatum (L.) Kraenzl. whole plant against CCl4 induced hepatotoxicity. Journal of Pharmacognosy and Phytochemistry. 2(3): 113-118.
Harborne,J.B. 1987. Metode Fitokimia : Penuntun Cara Modern Menganalisa Tumbuhan. Penerbit ITB. Bandung.
Hastari,R.2012. Uji aktivitas antibakteri ekstrak pelepah dan batang tanaman pisang Ambon (Musa paradisciaca var sapientum)terhadap Stapylococcus aureus.UNDIP, Semarang
Jalal, J.S., P. Kumar, L. Tewari, and YPS. Pangtey. 2010. Orchids: Uses in traditional medicine in India. National Seminar “Medicinal plants of Himalaya Potential and Prospect. India
Jorim, R.Y., S. Karape, W. Legu, M. Koch, L.R. Barrows, T.K. Matainboho, and P. Rai.2012. An ethnobotanical survey of medicinal plants used in the eastern highlands of Papua New Guinea. J.Ethnobiology Ethnomedicine 8 : 47.
Khan,H.,Marya, T. Belwal,M.Tariq,A.G.Atanasov, and H.P.Devkota.2019. Genus Vanda: A Review on traditional uses, bioactive chemical constituents and pharmacological activities. Journal of Ethnopharmacology 229:46-53
Kong, J.M., N.K. Goh, L.S. Chia, and T.F. Chin. 2003. Recent advances in traditional plant drug and Orchids. Acta Pharmacologica Sinica. 24(1): 7-21.
Meyer,BN.,Ferrigin,NR.,Putnam,JE, Jacobson,IB.,Nichols,DE,Melauhlin,JL.1982. BrineShrimp : A convenient general bioassay for active plant constituents. Planta Med. 45: 31-34.
Nugroho,L.H.,R. Pratiwi, R.Susandarini, E.R.P. Wardoyo, O. Megawati and S. Handayani. 2016. Isolation of bioactive compounds from two orchid species and preliminary test of their cytotoxicity against 747D breast cancer cells. International Journal of Pharmacognosy and Phytochemical Research 8(1):150-155
Panda, A.K. and D. Mandai. 2013. The folklore medicinal orchids of SIKKIM. Anc. Sci. Life. 33(2): 92–96.
Paudel,M.R., N. Rajbanshi, A.K. Sah, S. Acharya and B. Pant. 2018. Antibacterial activity of selected Dendrobium species against clinically isolated multiple drug resistant bacteria. African Journal of Microbiology Research 12 (18): 426-432.
Paul, P., A. Chowdhaury, A.D. Nath and M.K. Bhattacharjee. 2013. Antimicrobial efficacy of orchid extracts as potential inhibitors of antibiotic resistant strains of Escherichia coli. Asian Journal of Pharmaceutical and Clinical Research 6(3): 108-111.
Prasad,G., A.A. Mao, D. Vijayan, S. Mandal, K. Chandhuri, and T. Seal. 2016. Comparative HPLC fingerprinting and antioxidant activities of in vitro and in vivo grown Aerides odorata, a medicinal orchids. J. Chem. Bio. Phy. Sci. Sec. B. 6(2): 454-468.
Rashmi, K., S.D. Shweta, C.S. Sydeshna, P.S. Vrushala, TRP. Kekuda, and H.L. Raghavendra. 2015. Antibacterial and radical scavenging activity of selected orchids of Karnataka, India. Science, Technology and Arts Research Journal 4(1):160-164.
Sandrasagaran, U.M., S. Subramaniam, and V. Murugaiyah. 2014. New perspective of Dendrobium crumenatum orchid for antimicrobial activity against selected pathogenic bacteria. Pak. J. Bot. 46(2):719-724.
Widiatoety,D.,Nina S., dan Muchtar, S. 2010. Potensi anggrek Dendrobium dalam meningkatkan variasi dan kualitas anggrek bunga potong. Jurnal Litbang Pertanian 29(3): 101-106.
