Phytochemical Screening, Antibacterial and Cytotoxic Activity of Dendobium lasianthera J.J.Sm. from Papua
https://doi.org/10.31957/jbp.1289
Abstract
The aims of the study are to evaluate bioactive compounds, antibacterial and cytotoxic potential of D. lasianthera. This orchid grows well all over New Guinea Island as an ornamental plant because of their beautiful flowers. Orchids also known rich of its phytochemical compounds which already used as a traditional medicine in many countries around the world. However, research in pharmacological fields is still limited. In this study, leaves and stem of D. lasianthera were powdered and extracted with ethanol followed by fractionated using n-hexane, ethyl acetate, and ethanol solvent. Extract as well as fraction were tested for phytochemical screening and determined antibacterial activity against Escherichia coli and Staphylococcus aureus using Disc Diffusion Method. Brine Shrimp Lethality Test (BSLT) was used to observe cytotoxic potential of leaves and stem extract and fraction at 10, 50, 100, 250, 500, 750, and 1000 ppm. The results showed that the leaves and stem extract contained tannin and alkaloids, separately. The ethanol extract of D. lasianthera showed 7.35 mm (leaves) and 7.43 mm (stem) inhibition zone against S. aureus Furthermore, the maximum inhibition zone of ethanol fraction of leaves and n-hexane fraction of stem were 8.42 mm and 8.10 mm separately. The LC50 of stem extract and fractions in these study were 699.3 ppm (ethanol extract), 602.1 ppm (ethyl acetate), 329.6 ppm (n-hexane fraction) and 676 ppm (ethanol fraction), whereas for leaves, only ethyl acetate fraction has toxict activity with an average LC50 833.2 ppm.Â
Key words: D. lasianthera; phytochemical screening; antibacterial; cytotoxicity; Papua.Â
Downloads
References
Agustini, V., Suharyanto, Suharno, L. Dimara, and C.D. Sembay. 2013. The diversity of tropical orchids of South Papua. Jurnal Biologi Papua. 5(1): 1–9.
Agustini, V., S. Sufaati, dan Suharno. 2015. Anggrek dataran tinggi Habema di Distrik Nanggo, Trikora, Jayawijaya Papua. Jurnal Biologi Papua. 7(1): 9-15.
Behera, D., D.C.C. Rath,, and U. Mohapatra. 2013. Medicinal Orchids in India and their conservation : A Review. Floriculture and Ornamental Biotechnology 7(1):53-59.
Besra,R.C.,L.Majhee and J. Sharma. 2011. Evaluation of phytochemical,antioxidant and hepatoprotective activitu of tuber of Geodorum laxiflorum Griff. Journal of Pharmacology and Toxicology 6(7):610-623
Bulpitt, C.J., Y. Li., P.F. Bulpitt, and J. Wang. 2007. The use of orchids in Chinese medicine. Journal of the Royal Society of Medicine. 100: 558-563.
Cahyo, F. A and D Dinarti. 2015. pengaruh iradiasi sinar gamma terhadap pertumbuhan protocorm like bodies anggrek D. lasianthera (JJ. Smith) secara in vitro. J. Hort Indonesia. Vol 6(3): 177-186
Davis,WW and Stout,TR.1971. Disc plate method of microbiological antibiotic assay : factors influencing variability and error 1. Appl Microbiol:22 ($):659-665
Devi, P.U., S. Selvi, D. Devipriya, S. Murugan, and S. Suja. 2009. Antitumor and antimicrobial activities and inhibition of in vitro lipid peroxidation by Dendrobium nobile. African Journal of Biotechnology. 8 (10) : 2289-2293.
Ganapaty, S., M. Ramaiah, K. Yasaswini, V. K. Nuthakki, D. Harikrishnareddy. 2013. Quantitative phytochemical estimation and evaluation of hepatoproytective activity of methanolic extract of Dendrobium ovatum (L.) Kraenzl. whole plant against CCl4 induced hepatotoxicity. Journal of Pharmacognosy and Phytochemistry. 2(3): 113-118.
Harborne,J.B. 1987. Metode Fitokimia : Penuntun Cara Modern Menganalisa Tumbuhan. Penerbit ITB. Bandung.
Hastari,R.2012. Uji aktivitas antibakteri ekstrak pelepah dan batang tanaman pisang Ambon (Musa paradisciaca var sapientum)terhadap Stapylococcus aureus.UNDIP, Semarang
Jalal, J.S., P. Kumar, L. Tewari, and YPS. Pangtey. 2010. Orchids: Uses in traditional medicine in India. National Seminar “Medicinal plants of Himalaya Potential and Prospect. India
Jorim, R.Y., S. Karape, W. Legu, M. Koch, L.R. Barrows, T.K. Matainboho, and P. Rai.2012. An ethnobotanical survey of medicinal plants used in the eastern highlands of Papua New Guinea. J.Ethnobiology Ethnomedicine 8 : 47.
Khan,H.,Marya, T. Belwal,M.Tariq,A.G.Atanasov, and H.P.Devkota.2019. Genus Vanda: A Review on traditional uses, bioactive chemical constituents and pharmacological activities. Journal of Ethnopharmacology 229:46-53
Kong, J.M., N.K. Goh, L.S. Chia, and T.F. Chin. 2003. Recent advances in traditional plant drug and Orchids. Acta Pharmacologica Sinica. 24(1): 7-21.
Meyer,BN.,Ferrigin,NR.,Putnam,JE, Jacobson,IB.,Nichols,DE,Melauhlin,JL.1982. BrineShrimp : A convenient general bioassay for active plant constituents. Planta Med. 45: 31-34.
Nugroho,L.H.,R. Pratiwi, R.Susandarini, E.R.P. Wardoyo, O. Megawati and S. Handayani. 2016. Isolation of bioactive compounds from two orchid species and preliminary test of their cytotoxicity against 747D breast cancer cells. International Journal of Pharmacognosy and Phytochemical Research 8(1):150-155
Panda, A.K. and D. Mandai. 2013. The folklore medicinal orchids of SIKKIM. Anc. Sci. Life. 33(2): 92–96.
Paudel,M.R., N. Rajbanshi, A.K. Sah, S. Acharya and B. Pant. 2018. Antibacterial activity of selected Dendrobium species against clinically isolated multiple drug resistant bacteria. African Journal of Microbiology Research 12 (18): 426-432.
Paul, P., A. Chowdhaury, A.D. Nath and M.K. Bhattacharjee. 2013. Antimicrobial efficacy of orchid extracts as potential inhibitors of antibiotic resistant strains of Escherichia coli. Asian Journal of Pharmaceutical and Clinical Research 6(3): 108-111.
Prasad,G., A.A. Mao, D. Vijayan, S. Mandal, K. Chandhuri, and T. Seal. 2016. Comparative HPLC fingerprinting and antioxidant activities of in vitro and in vivo grown Aerides odorata, a medicinal orchids. J. Chem. Bio. Phy. Sci. Sec. B. 6(2): 454-468.
Rashmi, K., S.D. Shweta, C.S. Sydeshna, P.S. Vrushala, TRP. Kekuda, and H.L. Raghavendra. 2015. Antibacterial and radical scavenging activity of selected orchids of Karnataka, India. Science, Technology and Arts Research Journal 4(1):160-164.
Sandrasagaran, U.M., S. Subramaniam, and V. Murugaiyah. 2014. New perspective of Dendrobium crumenatum orchid for antimicrobial activity against selected pathogenic bacteria. Pak. J. Bot. 46(2):719-724.
Widiatoety,D.,Nina S., dan Muchtar, S. 2010. Potensi anggrek Dendrobium dalam meningkatkan variasi dan kualitas anggrek bunga potong. Jurnal Litbang Pertanian 29(3): 101-106.
Downloads
Published
Issue
Section
License
1. Copyright Policy
Jurnal Biologi Papua is committed to promoting the dissemination, accessibility, and responsible reuse of scholarly research in biology and related disciplines.
Under this policy, authors retain copyright ownership of their published articles and grant Jurnal Biologi Papua the non-exclusive right of first publication.
Authors retain the right to use, distribute, reproduce, and share their scholarly work in accordance with the applicable publication license.
The journal acknowledges the intellectual contributions of authors and respects their moral rights, including the right to be properly identified as creators of their work.
2. Publication License
Articles published under this policy are distributed under the Creative Commons Attribution 4.0 International License (CC BY 4.0).
License URL: https://creativecommons.org/licenses/by/4.0/
Under this license, users are permitted to:
-
Share: Copy and redistribute published articles in any medium or format.
-
Adapt: Remix, transform, and build upon published materials for any purpose, including commercial purposes.
-
Redistribute: Make articles available through repositories, websites, databases, and other scholarly platforms.
These permissions are subject to the Creative Commons Attribution 4.0 International License.
Users must provide appropriate credit to the original authors, include a link to the license, and indicate whether changes have been made.
Reuse must not imply endorsement by the authors or publisher.
3. Rights of Authors
Authors publishing in Jurnal Biologi Papua retain the following rights:
-
Copyright ownership of their original scholarly work.
-
The right to distribute and share their published articles.
-
The right to deposit their articles in institutional or subject repositories.
-
The right to reuse their work in subsequent scholarly publications, subject to appropriate acknowledgment and applicable ethical standards.
-
The right to share their work for teaching, research, academic collaboration, and other lawful purposes.
Authors must acknowledge the original publication in Jurnal Biologi Papua when distributing or reusing the published version.
4. Rights of the Publisher
Authors grant Jurnal Biologi Papua the non-exclusive right to publish, disseminate, archive, and preserve accepted manuscripts.
The journal may reproduce and distribute the published version through its official website and authorized indexing, archiving, and scholarly dissemination services.
The publisher is responsible for maintaining the integrity of published content and accurately displaying copyright and licensing information.
5. Open Access Policy
Jurnal Biologi Papua supports immediate open access to scholarly research.
Articles published under this policy are freely accessible to readers without subscription requirements or access fees.
The journal encourages the responsible dissemination and reuse of published research to support scientific progress, academic collaboration, and the broader exchange of knowledge.
Published content may be accessed, downloaded, shared, and reused in accordance with the applicable Creative Commons license.
6. Third-Party Materials
Authors are responsible for ensuring that all materials included in their manuscripts comply with applicable copyright and intellectual property laws.
Permission must be obtained when required for the use of third-party figures, photographs, tables, illustrations, or other copyrighted materials.
Materials excluded from the article's Creative Commons license must be clearly identified with their applicable copyright and permission notices.
The journal's publication license does not override the rights of third-party copyright holders.
7. Repository and Self-Archiving Policy
Authors are permitted and encouraged to deposit and share their published articles through institutional repositories, academic repositories, and personal or institutional websites.
Authors must provide appropriate acknowledgment of the article's original publication in Jurnal Biologi Papua, including its citation information and DOI where available.
Repository deposits and subsequent distribution must comply with the license applicable to the relevant version of the article.
8. Licensing Information and Article Metadata
The applicable copyright holder and publication license should be clearly identified on each published article's webpage.
Where supported, licensing information should also be included in article metadata and the published PDF.
The journal is committed to maintaining accurate and consistent licensing information across its online publishing platform and scholarly indexing services.
9. Policy Application and Previously Published Articles
This policy applies to manuscripts accepted following its formal adoption by the journal and publisher.
Articles published before the adoption of this policy remain subject to the copyright arrangements and licenses applicable at the time of their publication, unless a valid change is authorized by the relevant rights holders.
The journal will not apply a new license retroactively without the necessary authorization.
10. Policy Commitment
Jurnal Biologi Papua is committed to transparent copyright arrangements, responsible open access publishing, and respect for intellectual property rights.
The journal will periodically review its copyright and licensing practices to maintain consistency with recognized standards of scholarly communication.
References
Creative Commons Attribution 4.0 International License: https://creativecommons.org/licenses/by/4.0/
Directory of Open Access Journals (DOAJ): https://doaj.org/apply/copyright-and-licensing/
