Analisis Total Kadar Asam Laktat Hasil Fermentasi Pare (Momordica charantia) Secara Spontan dengan Konsentrasi Garam Berbeda

Putri S. Noor; Wijanarka Wijanarka

doi:10.31957/jbp.1642

Authors

Putri S. Noor Student of Diponegoro University https://orcid.org/0000-0003-4515-4786
Wijanarka Wijanarka Diponegoro University

DOI:

https://doi.org/10.31957/jbp.1642

Abstract

Bitter melon (Momordica charantia) is a traditional medicinal plant with great potential for treating diabetes due to its ability to lower blood sugar level. The bitter taste of this plant is attributed to its high nutrient and antioxidants content. Meanwhile, one of the ways to reduce the bitterness and improve its taste is through a spontaneous fermentation process with the right salt concentration.Â Therefore, this study aims to analyze the effect of different salt concentrations on the total lactic acid content of spontaneous bitter melon fermentation. This study was conducted using a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments used included salt of different concentrations, namely: 3, 6, 9, and 12%. The bitter melon was fermented for 7 days and the parameter measured was the total lactic acid content through titration process. Furthermore, data were analyzed by ANOVA with 5% significance and the Duncan test was also carried out. The results showed different salt concentrations had a significant effect on lactic acid contents (P <0.05), with the highest being 0.4413% produced by 3% salt concentration treatment. These results are expected to contribute to the development of bitter melon utilization, as well as provide solutions, therefore, it becomes more effective and efficient food processing.Â Â

Key words: bitter melon; fermentation; lactic acid; salt; spontaneous.

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Author Biographies

Putri S. Noor, Student of Diponegoro University

Biotechnology major, Department of Biology, Faculty of Science and Mathemathic, Diponegoro University

Wijanarka Wijanarka, Diponegoro University

Biotechnology, Department of Biology, Faculty of Science and Mathemathic, Diponegoro University

References

Adnyana, I.D.P.A., D.K. Meles, Wurlina, S. Zakaria, dan N. Suwasanti. 2016. Efek Antidiabetes Buah Pare (Momordica charantia Linn.) Terhadap Kadar Glukosa Darah, Sel Penyusun Pulau Langerhans dan Sel Leydig pada Tikus Putih Hiperglikemia. Acta Veterinaria Indonesiana. 4(2): 43-50.

Ahillah, N., A. Rusdanillah, W. Afiana, R. Sulistiani, dan R.P.L. Mail. 2017. Pengaruh Konsentrasi Garam pada Fermentasi Ikan Wader (Rasbora lateristriata). Bioedukasi. 10(2): 12-17.

Basch, E., S. Gabardi, and C. Ulbricht. 2003. Bitter melon (Momordica charantia): Review of Efficacy and Safety. Am J Health Syst Pharm. 60(4): 356-359.

Cappuccino, J.G., and C.T. Welsh. 2019. Microbiology: A Laboratory Manual 12th ed. Pearson. New York.

Ghaffar, T., M. Irshad, Z. Anwar, T. Aqil, Z. Zulifqar, A. Tariq, M. Kamran, N. Ehsan, and S. Mehmood. 2014. Recent Trends in Lactic Acid Biotechnology: A Brief Review on Production to Purification. Journal of Radiation Research and Applied Sciences. 7(2): 222-229.

Joseph, B. and D. Jini. 2013. Antidiabetic Effects of Momordica charantia (Bitter Melon) and Its Medicinal Potency. Asian Pac J Trop Dis. 3(2): 93-102.

Joshi, V.K. and S. Sharma. 2009. Lactic Acid Fermentation of Radish For Shelf Stability And Pickling. Natural Product Radiance. 8(1): 19-24.

Lemi, B.W. 2020. Microbiology of Ethiopian Traditionally Fermented Beverages and Condiments. International Journal of Microbiology. doi: 10.1155/2020/1478536.

Mazlan, F.A., M.S.M. Annuar and Y. Sharifuddin. 2015. Biotransformation of Momordica charantia Fresh Juice by Lactobacillus plantarum BET003 and Its Putative Anti-Diabetic Potential. PeerJ, 1-18. doi: 10.7717/peerj.1376.

Nurhalimah, N., B. Sulistiyanto dan Widiyanto. 2015. Kandungan Bakteri Asam Laktat dan Bakteri Selulolitik pada Pollard yang Difermentasi. [Skripsi]. Universitas Diponegoro, Semarang.

Nurjannah, L., Suryani, S.S. Achmadi, and A. Azhari. 2017. Produksi Asam Laktat oleh Lactobacillus delbrueckii subsp. bulgaricus dengan Sumber Karbon Tetes Tebu. Jurnal Teknologi dan Industri Pertanian Indonesia. 9(1): 1â€“9.

Ramadzanti, A. 2006. Aktvitas Protease dan Kandungan asam Laktat pada Yoghurt yang dimodifikasi Bifidobacterium bifidum. [Skripsi]. Institut Pertanian Bogor, Bogor.

Riyadi, N.H., D. Ishartani, dan R. Purbasari. 2015. Mengangkat Potensi Pare (Momordica Charantia) Menjadi Produk Pangan Olahan sebagai Upaya Diversifikasi. Pros Sem Nas Masy Biodiv Indon. 1(5): 1167-1172.

Rolle, R. and M. Satin. 2002. Basic Recruitments For The Transfer Of Fermentation Technologies To Developing Countries. International Journal of Food Microbiology. 75(3): 181-187.

Ross, R.P., S. Morgan, and C. Hill. 2002. Preservation and Fermentation: Past, Pesent, and Future. International Journal of Food Microbiology. 79(1-2): 3-16.

Rukmana, R. 1997. Budidaya Pare. Kanisius. Yogyakarta.

Setiawan, N. Yuliana, dan S. Setyani. 2013. Pengaruh Konsentrasi Garam Terhadap Warna, Total Asam dan Total Bakteri Asam Laktat Pikel Ubi Jalar Ungu (Ipomoea batatas var Ayamurasaki) Selama Fermentasi. Jurnal Teknologi Industri dan Hasil Pertanian. 18(1): 42-51.

Shobahiya, N. 2017. Pengaruh Jenis Media Fermentasi dan Konsentrasi Garam Terhadap Karakteristik Asinan Sawi Hijau (Brassica rapa L). [Skripsi]. Universitas Pasundan, Bandung.

Shockey, K.K. and C. Shockey. 2014. Fermented Vegetables: Creative Recipes for Fermenting 64 Vegetables & Herbs in Krauts, Kimchis, Brined Pickles, Chutneys, Relishes & Pastes. Storey Publishing. Massachusetts.

Silva, G.M.S.W., U.L.R.R.W. Premathilaka, S.D.T. Maduwanthi, and T.G.G. Uthpala. 2016. Development of Fermented Momordica charantia and Analysis of Biochemical Properties. International Journal of Scientific & Engineering Research. 7(3): 363-366.

Snee, L.S., V.R. Nerurkar, D.A. Dooley, J.T. Efird, A.C. Shovic, and P.V. Nerurkar. 2011. Strategies To Improve Palatability And Increase Consumption Intentions For Momordica charantia (Bitter Melon): A Vegetable Commonly Used For Diabetes Management. Nutr J. 10(1): 1-11.

So'aib, M.S., K.H.K. Hamid, J. Salihon, H.L. Tan, and A. Hamid. 2020. Beneficial Effects Of Spontaneous Fermentation In Enhancing The Phenolic Contents, Antioxidant Activity And Cultivation Of Lactic Acid Bacteria Of Carica papaya Leaf. IOP Conf. Series: Mater. Sci. Eng. 991(1). doi:10.1088/1757-899X/991/1/012006

Soetiarso, T.A. 2010. Persepsi dan Preferensi Konsumen Terhadap Atribut Produk Beberapa Jenis Sayuran Minor. J. Hort. 20(3): 299-312.

Swain, M.R., M. Anandharaj, R.C. Ray, and R.P. Rani. 2014. Fermented Fruits and Vegetables of Asia: A Potential Source of Probiotics. Biotechnology Research International. doi: 10.1155/2014/250424.

Touret, T., M. Oliveira, and T. Semedo-Lemsaddek. 2018. Putative Probiotic Lactic Acid Bacteria Isolated from Sauerkraut Fermentations. PLoS ONE. 13(9): 1-16.