Bakteri Indigen yang Diisolasi dari Danau Toba: Acinetobacter junii DT2.1 Multiresisten Tembaga, Methylen Blue, dan Basic Fuchine

https://doi.org/10.31957/jbp.2804

Authors

  • Wahyu Irawati Universitas Pelita Harapan
  • Polin P. Ambarita Universitas Pelita Harapan
  • Vannesa El Shaday Universitas Pelita Harapan
  • Sintia Y. Rahmawati Universitas Pelita Harapan

Keywords:

indigenous bacteria, Lake Toba, multi-resistence, dye, copper

Abstract

Lake Toba is one of the industrial waste disposal sites around the location. Copper and dyes are pollutants that are toxic and harmful to organisms. Bioremediation using indigenous bacteria isolated from polluted sites is a promising solution to overcome environmental pollution. Bacteria that have multi-resistance to copper and dyes are expected to increase the efficiency of industrial waste treatment. This study aims to: 1) isolate and characterize copper-resistant bacteria, 2) determine the level of resistance of bacterial isolates to copper, 3) test the resistance of bacteria to dyes. Physiological characterization of bacterial isolates was carried out by observing colony and cell morphology, also Gram staining. Molecular characterization was carried out by analysis of the 16S rDNA gene. The copper resistance level was tested by determining the Minimum Inhibitory Concentration MIC value. The dye resistance test was carried out by growing bacteria on a medium containing 100 ppm of dye. There were eight isolates of copper-resistant bacteria with MIC values ranging from 3-7 mM CuSO4 with code as DT1.1; DT1.2; DT1.3; DT1.4; DT1.5; DT2.1; DT2.2; and DT3.1. The three highly resistant bacteria were strains DT1.1 and DT1.3 which were identified as Lysinibacillus macroides and strain DT2.1 was identified as Acinetobacter junii with an MIC value of 7mM CuSO4, respectively. The results of the resistance test to dyes showed that only A. junii strain DT2.1 was resistant to 100 ppm Methylene Blue and Basic Fuschine. 

Key words: indigenous bacteria; Lake Toba; multi-resistence; dye; copper.

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References

Buaton, K.W., dan H. Purwadio. 2015. Kriteria pengembangan kawasan wisata Danau Toba Parapat, Sumatera Utara. Jurnal Teknik ITS. 4(1): 1–5.

Cahyani, M., R. Azizah, dan B. Yulianto. 2012. Studi kandungan logam berat tembaga (Cu) pada air, sedimen, dan kerang darah (Anadara granosa) di perairan Sungai Sayung dan Sungai Gonjol, Kecamatan Sayung, Kabupaten Demak. Journal of Marine Research. 1(2): 73-79.

Ed-Har, A.A., R. Widyastuti, dan G. Djajakirana. 2017. Isolasi dan identifikasi mikroba tanah pendegradasi selulosa dan pektin dari rhizosfer Aquilaria malaccensis. Buletin Tanah dan Lahan. 1(1): 58–64.

Noer, S. 2021. Identifikasi Bakteri secara molekular menggunakan 16S rRNA. EduBiologia: Biological Science and Education Journal. 1(1): 1. DOI: doi.org/10.30998/edubiologia.v1i1.8596.

Garno, Y.S., R. Nugroho, dan M. Hanif. 2020. Kualitas air Danau Toba di wilayah Kabupaten Toba Samosir dan kelayakan peruntukannya. Jurnal Teknologi Lingkungan. 21(1): 118-124.

Gupta, R., T. Kumar, and A. Mittal. 2015. Isolation, identification and characterization of heavy metal resistant bacteria from soil of an iron industry, Haryana (India). Intl J Pharm Sci Res. 7(3): 1308-1313.

Hamdaoui, O., and M. Chiha. 2006. Removal of methylene blue from aqueous solution by wheat bran. Acta Chimica Slovenia. 54: 407–418.

Harianja, D., M. Damanik, dan R. Restu. 2018. Kajian tingkat pencemaran air di kawasan perairan Danau Toba Desa Silima Lombu Kecamatan Onanrunggu Kabupaten Samosir. Jurnal Geografi. 10(2): 176–183.

Hobman, J.L., and L.C. Crossman. 2014. Bacterial antimicrobial metal ion resistance. J Med Microbiol. 64: 471-497.

Irawati, W., A. Hasthosaputro, dan L. Kusumawati. 2020. Multiresistensi dan akumulasi Acinetobacter sp. IrC2 terhadap logam berat. Jurnal Biologi Papua. 114-122.

Irawati, W., N. Ompusunggu, D. Susilowati, and T. Yuwono. 2019. Molecular and physiological characterization of indigenous copperresistant bacteria from Cikapundung River, West Java, Indonesia. Biodiversitas. 20(2): 344-349.

Irawati, W., R. Pinontoan, B. Mouretta, and T. Yuwono. 2022. The potential of copper-resistant bacteria Acinetobacter sp. strain Cn5 in decolorizing dyes. Biodiversitas. 23(2): 680-686.

Irawati, W., E.S. Djojo, L. Kusumawati, T. Yuwono, and R. Pinontoan. 2021. Optimizing bioremediation: Elucidating copper accumulation mechanisms of Acinetobacter sp. IRC2 isolated from an industrial waste treatment center. Front Microbiol. 12: 713812. DOI: 10.3389/fmicb.2021.713812.

Irawati, W., S. Riak, N. Sopiah, and S. Sulistia. 2017. Heavy metal tolerance in indigenous bacteria isolated from the industrial sewage in Kemisan River, Tangerang, Banten, Indonesia. Biodiversitas. 18(4): 1481-1486.

Khandare, R.V., and S.P. Govindwar. 2016. Microbial degradation mechanism of textile dye and its metabolic pathway for environmental safety. In: Environmental Waste Management. CRC Press.

Komarawidjaja, W. 2016. Sebaran limbah cair industri tekstil dan dampaknya di beberapa desa Kecamatan Rancaekek Kabupaten Bandung. Jurnal Teknologi Lingkungan. 17(2): 118–125.

Li, C., Y. Li, and C. Ding. 2019. The role of copper homeostasis at the host-pathogen axis: From bacteria to fungi. Int J Mol Sci. 20(1): 175-182.

Lukman. 2013. Danau Toba: Karakteristik limnologis dan mitigasi ancaman. LIPI Press. Jakarta.

Méndez, V., S. Fuentes, V. Morgante, M. Hernández, M. González, E. Moore, and M. Seeger. 2017. Novel hydrocarbonoclastic metal-tolerant Acinetobacter and Pseudomonas strains from Aconcagua river oil polluted soil. J Soil Sci Plant Nutr. 17(4): 1074-1087.

Naimah, S., S. Ardhanie, B. Jati, N. Aidha, dan A. Arianita. 2014. Degradasi zat warna pada limbah cair industri tekstil dengan metode fotokatalitik menggunakan nanokomposit Tio2–Zeolit. Balai Besar Kimia dan Kemasan, Kementerian Perindustrian Ri. 225.

Noer, S. 2021. Identifikasi bakteri secara molekular menggunakan 16S rRNA. EduBiologia: Biological Science and Education Journal. 1(1): 1. DOI: doi.org/10.30998/ edubiologia.v1i1.8596.

Sekarwati, N., B. Murachman, dan Sunarto. 2015. Dampak logam berat Cu (tembaga) dan Ag (perak) pada limbah cair industri perak terhadap kualitas air sumur dan kesehatan masyarakat serta upaya pengendaliannya di Kota Gede Yogyakarta. Jurnal Ekosains. 7(1): 64-76.

Silaban, W., dan M. Silalahi. 2021. Analisis kualitas air di perairan Danau Toba Kecamatan Pangururan, Kabupaten Samosir. Jurnal Sains dan Teknologi. 10(2): 299-307.

Sugiyono. 2014. Metode penelitian kuantitatif, kualitatif dan R&D. Penerbit Alfabeta. Bandung.

Syahroni, C., dan Djarwanti. 2015. Pengembangan reaktor fotokatalitik rotating drum untuk pengolahan air limbah industri tekstil. Jurnal Riset Teknologi Pencegahan Pencemaran Industri. 6(2): 35–44.

Tampubolon, H.S., D. Bakti, dan I. Lesmana. 2013. Studi kandungan logam berat tembaga (Cu) dan timbal (Pb) di perairan Danau Toba, Provinsi Sumatera Utara. [Thesis]. Universitas Sumatera Utara.

Tortora, G.J., D.R. Funke, and C.L. Case. 2015. Microbiology: An introduction. 8th Ed. Benjamin Cummings, San Fransisco.

Williams, C.L., H.M. Neu, J.J. Gilbreath, S.L.J. Michel, D.V. Zurawski, and D.S. Merrella. 2016. Copper resistance of the emerging pathogen Acinetobacter baumannii. Appl Environ Microb. 82(20): 6174-6188.

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Published

2023-10-01

Issue

Vol. 15 No. 2 (2023)

Section

Research Articles

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