Keragaman Fungi Mikoriza Arbuskula Pada Tumbuhan Pokem [Setaria italica (L.) Beauv.] dengan Metode Traping
DOI:
https://doi.org/10.31957/jbp.437Abstract
Arbuscular Mycorrhizal Fungi (AMF) plays important role in increasing plant growth and plant protection against pathogen. Its diversity depends on their habitat, including the abundance and distribution of the host plant on agricultural area. This research has aim to explore the diversity of AMF from the rhizosphere of Pokem (Setaria italica) using trapping methods. Soil samples were collected from Numfor Island, Biak Numfor, Papua. The isolation of AMF from the soil sample was elucidated by wet sieving method. Every different spore then was inoculated to the root of Desmodium heterocarpon to propagate and monosporic culture was grown on Shorgum bicolor.  The result showed that the number of the spore that was obtained by traping method increased up to 63,63%. Single spores inoculation of AMF on D. heterocarpon was high percentage (85%) of success. On average, Monosporic culture of single spore inoculation on S. bicolor reached up to 30,53 spores/10g soil sample.  There are 6 genera (9 species) were obtain in this study, namely Glomus, Claroideoglomus, Scutellospora, Gigaspora, Ambispora and Acaulospora. Furthermore, identification using molecular method should be done for proper identification.
Key words: AMF, plant rhizosfer, S. italica, trapping methods
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References
Abbott, L.K. and A.D. Robson. 1982. The role of vesicular arbuscular mycorrhizal fungi in agriculture and the selection of fungi for inoculation. Australian Journal of Agricultural Research. 33(2): 389–408.
Ãlvarez-Sánchez, J., N.C. Johnson, A. Antoninka, V.B. Chaudhary, M.K. Lau, S.M. Owen, I. Sánchez-Gallen, P. Guadarrama and S. Castillo. 2012. Large-scale diversity patterns in spore communities of arbuscular mycorrhizal fungi. In: Mycorrhiza: Occurrence in Natural and Restored (M. Pagano, Ed.). Nova Science Publishers, Inc. New York, USA.
Anju, T. dan S. Sarita. 2010. Suitability of foxtail millet (Setaria italica) and barnyard millet (Echinochloa frumentacea) for development of low glycemic index biscuits. Malays. J. Nutr. 16(3): 361–368.
Brundrett, M. 1991. Mycorrhizas in natural ecosystem. Advances in Ecological Research. 21: 171–313.
Brundrett, M.C., Y. Piche, and R.L. Peterson. 1984. A new method for observing the morphology of vesicular arbuscular mycorrhizae. Can. J. Bot. 62: 2128–2134.
Cuenca, G. and M. Lovera. 2010. Seasonal variation and distribution at different soil depths of arbuscular mycorrhizal fungi spores in a tropical sclerophyllous shrubland. Botany. 88: 54–64.
Daisog, H., C. Sbrana, C. Cristani, A.-C. Moonen, M. Giovnnetti and P. Barbaeri. 2012. Arbuscular mycorrhizal fungi shift competitive relationships among crop and weed species. Plant Soil. 353: 395–408.
Dodd, J.C., J.P. Clapp, and B. Zhao. 2001. Mycorrhiza Manual. prepared for the Workshop. Arbuscular mycorrhizal fungi in plant production systems: detection, taxonomy, conservation and ecophysiology. Laboratory of Agricultural Microbiology, Huazhong Agricultural University. China.
Garg, N and S. Chandel. 2010. Arbuscular mycorrhizal networks: Process and function. A review. Agron. Sustain. Dev. 30: 581–599.
Hubbard, F.T. 1915. A Taxonomic study of Setaria italica and its immediate allies. American Journal of Botany. 2(4): 169–198.
IJdo, M., S. Cranenbrouck and S. Declerck. 2011. Methods for large–scale production of AM fungi: past, present, and future. Mycorrhiza. 21:1–16.
Jansa, J., A. Mozafar, T. Anken, R. Ruh, I.R. Sanders and E. Frossard. 2002. Diversity and structure of AMF communities as affected by tillage in a temperate soil. Mycorrhiza. 12: 225–234.
Krishnamoorthy, R., C.-G. Kim, P. Subramanian, K.-Y. Kim, G. Selvakumar, and T.-M. Sa. 2015. Arbuscular Mycorrhizal Fungi Community Structure, Abundance and Species Richness Changes in Soil by Different Levels of Heavy Metal and Metalloid Concentration. PLoS ONE. 10(6): e0128784.
Malik, A. 2012. Pokem Setaria italica L. sumber pangan alternatif di masa datang. http://papua.litbang.deptan. go.id/ind/images/Document/pokem.pdf. (Download 2012).
Mangay, A.S., W.N. Pearson, and W.J. Darby. 1957. Millet (Setaria italica): its amino acid and niacin content and supplementary nutritive value for corn (maize) foxtail millet (Setaria Italica L.). Suplementary Value of Millet. 377–390.
Moreira, M., M.A. Nogueira., S.M. Tsai., S.M. Gomes-da-Costa., and E.J.B.N. Cardoso. 2007. Sporulation and diversity of arbuscular mycorrhizal fungi in Brazil Pine in the field and in the greenhouse. Mycorrhiza. 17: 519–526.
Morton, J., S. Bentivenga, and J. Bever. 1995. Discovery, measurement, and interpretation of diversity in arbuscular endomycorrhizal fungi (Glomales, Zygo-mycetes). Can J. Bot. 73: S25–S32.
Peterson, R.L., H.B. Massicotte, and L.H. Melville. 2010. Mycorrhizas: Anatomy and Cell Biology. CAB International, Wallingford, Oxon. United Kingdom.
Rauf, A.W., dan M.S. Lestari. 2009. Pemanfaatan komoditas pangan lokal sebagai sumber pangan alternatif di Papua. Jurnal Litbang Pertanian. 28(2): 54–62.
Schüßler, A. and C. Walker. 2010. The glomeromycota. A species list with new families and new genera. With correction on July 2011. [online]. The Royal Botanic Garden Kew, Botanische Staatssammlung Munich, and Oregon State University.
Smith, S.E., and D. Read. 2008. Mycorrhizal symbiosis. Third Edition. Academic Press, Elsevier. New York.
Sufaati, S., V. Agustini, R.H.R. Tanjung, dan Suharno. 2014. Keberadaan fungi mikoriza arbuskula pada gandum “Pokem†Papua [Setaria italica (L.) Beauv.] di Pulau Numfor, Kabupaten Biak Numfor. Seminar Nasional Biologi Indonesia. Jayapura, 7–8 Oktober 2014. pp: 61–69.
Suharno dan R.P. Sancayaningsih. 2013. Fungi Mikoriza Arbuskula: Potensi teknologi mikorizoremediasi logam berat dalam rehabilitasi lahan tambang. Bioteknologi. 10(1): 31–42.
Suharno, R.P. Sancayaningsih, E.S. Soetarto dan R.S. Kasiamdari. 2014. Keberadaan fungi mikoriza arbuskula di kawasan tailing tambang emas Timika sebagai upaya rehabilitasi lahan ramah lingkungan. J. Manusia dan Lingkungan. 21(3): 295–303.
Suharno, S. Sufaati, V. Agustini, dan C. Irawan. 2012. Fungi mikoriza arbuskula yang berasosiasi dengan gulma di lahan pertanian Koya Barat, Kota Jayapura, Papua. Prosiding Seminar Nasional Mikologi dan Pembentukan Perhimpunan Mikologi Indonesia. Purwokerto, 15–15 Mei 2012.
Suharno, S. Sufaati, V. Agustini, dan R.H.R. Tanjung. 2015. Usaha domestifikasi tumbuhan pokem (Setaria italica L.) masyarakat lokal Pulau Numfor, Kabupaten Biak Numfor sebagai upaya menunjang ketahanan pangan nasional. Jurnal Manusia dan Lingkungan. 22(1): 73–83.
Symanczik, S., J. Błaszkowski, S. Koegel, T. Boller, A. Wiemken, M.N Al-Yahya'ei. 2014. Isolation and identification of desert habituated arbuscular myco-rrhizal fungi newly reported from the Arabian Peninsula. J. Arid Land. 6(4): 488–497.
Turk, M.A., T.A. Assaf, K.M. Hameed and A.M. Al-Tawaha. 2006. Significance of mycorrhizae. World Journal of Agricultural Sciences. 2 (1): 16–20.
Vierheilig, H., P. Schweiger and M. Brundrett. 2005. An overview of methods for the detection and observation of arbuscular mycorrhizal fungi in roots. Physiologia Plantarum. 125: 393–404.
Walker, C. 2008. Mycological Research News. Mycological Research. 112: 297–298.
Wu, Q.-S., G.-H. Li and Y.N. Zou. 2011. Roles of arbuscular mycorrhizal fungi on growth and nutrient acquisition of peach (Prunus persica L. Batsch) seedlings. The Journal of Animal & Plant Sciences. 21(4): 746–750.
