Quantitative Analysis of Flavonoid Content, Total Phenolic and Toxicity Test of Extract Smilax rotundifolia Leaves From Papua
https://doi.org/10.31957/jbp.4539
Keywords:
BSLT, Smilax rotundifolia , spectrophotometry , total flavonoids , total phenolicAbstract
This study investigates Bungkus leaf (Smilax rotundifolia), a well-known plant in Papua commonly referred to as the three-finger leaf. Indigenous Papuans traditionally use this plant to enlarge genitals, buttocks, and breasts, as well as to treat syphilis. The plant is known to contain flavonoid and phenolic compounds with pharmacological activity. This study aimed to determine the total flavonoid and total phenolic content of the 70% ethanol extract of Bungkus leaf. The research began with simplicia preparation, followed by extraction using 70% ethanol. Qualitative tests, including phytochemical screening and thin-layer chromatography (TLC), were conducted, followed by quantitative analysis using UV-Vis spectrophotometry. The results showed total flavonoid content of 54.696 mg QE/g ± 0.565 and total phenolic content of 32.908 mg GAE/g ± 0.263. The study concluded that the total flavonoid content was higher than the total phenolic content. Toxicity testing categorized the extract as toxic, with an LC50 value of 442.92 ppm.
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References
Agustina, R., G. Alam, and C. Lethe. 2017. Aktivitas antimitosis hasil fraksinasi ekstrak kloroform spons Siphanocalina sp terhadap sel zigot bulu babi Tripneustus gratilla Linn. Majalah Farmasi dan Farmakologi. 21(3): 59–62.
Akma, R.R., M.D. Sulain, D. Susanti, and W.R.W. Ishak. 2022. Traditional uses, pharmacology, toxicology and chemical constituents of an aphrodisiac plant Smilax myosotiflora: A systematic review Bir. Hacettepe University Journalo of The Faculty of Pharmacy. 42(4): 276-29.
Chang, C., M. Yang, H. Wen, and J. Chern. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drugs Analysis. 10(3): 178–182.
Defri, I., S. Palupi, S.T. Wahyudi, and D. Yuliana. 2022. Antioxidant analysis of kawa daun (Coffea canephora) beverage by in vitro and in silico approaches. Indonesian Journal of Chemistry. 22(2): 374-386.
Dewantara, L., A. Ananto, and Y. Andayani. 2021. Penetapan kadar fenolik total ekstrak kacang panjang (Vigna unguiculata) dengan metode spektrofotometri UV-Visible. Jurnal Ilmu Kefarmasian. 2(1): 13-19.
Harbone, J.B. 1987a. Comparative biochemistry of flavonoids. Academic Press. London.
Harborne, J.B. 1987b. Metode fitokimia penuntun cara modern menganalisa tumbuhan. Penerbit ITB. Bandung.
Hariana, H.A. 2013. 262 Tumbuhan obat dan khasiatnya. Penebar Swadaya Grup. Jakarta.
Ifadah, R.A., P.R.W. Wiratara, dan C.A. Afgani. 2022. Ulasan ilmiah: Antosianin dan manfaatnya untuk kesehatan. Jurnal Teknologi Pengolahan Pertanian. 3(2): 11-21.
Jonatan, T.M. 2016. Identifikasi senyawa alkaloid ekstrak bungkus leaves (Similax sp.) yang berasal dari Biak Papua. Penerbit ITB. Bandung.
Kementerian Kesehatan RI. 2017. Farmakope herbal Indonesia Edisi II. Kementrian Kesehatan RI. Jakarta.
Kumalasari, E., N.M. Nararia, and S. Musiam. 2021. Penetapan kadar fenolik total ekstrak etanol 70% dan fraksi etil asetat daun bawang dayak (Eleutherine palmifolia (L.) Merr) dengan metode spektrofometri UV-Vis. Jurnal Insan Farmasi Indonesia. 4(1): 74-84.
Kurniawan, H., and M. Ropiqa. 2021. Uji toksisitas ekstrak etanol daun ekor kucing (Acalypha hispida Burm.f.) dengan metode brine shrimp lethality test (BSLT). Journal Syifa Sciences and Clinical Research. 3(2): 52–62.
Mahfirohtun, Y. 2020. Penentuan kadar senyawa flavonoid ekstrak kombinasi buah anggur, tin, delima dan zaitun menggunakan analisis spektofotometer UV-Vis. [Skripsi]. UIN Maulana Malik Ibrahim. Malang.
Manopo, F.C., F. Fatimawali, and O. Datu. 2023. Phytochemical screening and toxicity test of mangrove leaf ethanol extract (Bruguiera gymnorrhiza) with Brine Shrimp Lethality Test (BSLT) method. Pharmacon. 12(1): 83-91.
Nur, S., F.J. Sami, A. Awaluddin, and M.I.A. Afsari. 2019. Korelasi antara kadar total flavonoid dan fenolik dari ekstrak dan fraksi daun jati putih (Gmelina arborea Roxb.) terhadap aktivitas antioksidan. Jurnal Farmasi Galenika. 5(1): 33-42.
Padamani, E., J. Ngginak, and A.T. Lema. 2020. Analisis kandungan polifenol pada ekstrak tunas bambu betung (Dendrocalamus asper). Bioma: Jurnal Biologi dan Pembelajaran Biologi. 5(1): 52-65.
Putri, D.R., L. Dharmayanti, and P.A. Nurwani. 2020. Skrining fitokimia dan penetapan kadar flavonoid ekstrak biji kesumba keling (Bixa orellana L). [Skripsi]. Stikes Al-Fatah Bengkulu. Bengkulu.
Putri, J.Y., K. Nastiti, and N. Hidayah. 2023. Pengaruh pelarut etanol 70% dan metanol terhadap kadar flavonoid total ekstrak daun sirsak (Annona muricata Linn): Pengaruh pelarut etanol 70% dan metanol terhadap kadar flavonoid total tkstrak daun sirsak (Annona muricata Linn). Journal Pharmaceutical Care and Sciences. 3(2): 20-29.
Rahayu, S., R. Vifta, and J. Susilo. 2021. Uji aktivitas antioksidan ekstrak etanol bunga telang (Clitoria Ternatea L.) dari Kabupaten Lombok Utara dan Wonosobo menggunakan metode FRAP. Journal of Research in Pharmacy. 1(2): 1-9.
Sunarti. 2021. Antioksidan dalam penanganan sindrom metabolik. UGM Press. Yogyakarta.
Swasana, A.R.N.W. 2019. Optimasi kadar flavonoid bubuk ekstrak kulit melinjo berwarna hijau (Kajian suhu dan lama pengeringan. [Skripsi]. Universitas Brawijaya. Malang.
Vifta, R.L., and Y.D. Advistasari. 2018. Skrining fitokimia, karakterisasi, dan penentuan kadar flavonoid total ekstrak dan fraksi-fraksi buah parijoto (Medinilla speciosa B.). Prosiding Seminar Nasional Unimus. 1(1): 8–14.
Wulandari, A., R. Patala, K.R. Handayani, and M.S. Makatang. 2022. Aktivitas afrodisiak ekstrak etanol daun tumbuhan bungkus (Smilax rotundifolia L.) terhadap fertilitas tikus putih jantan (Rattus norvegicus). Jurnal Riset Kimia. 8(3): 215–221.
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