Uji Resistensi Antibiotik dan Deteksi Gen Plasmid IncHI1 Salmonella typhi Isolat Jayapura
DOI:
https://doi.org/10.31957/jbp.47Abstract
The research on antibiotic resistance test and detection of gene Plasmid IncHI1 1 Salmonella typhi isolates of Jayapura have been conducted in January to August 2015. The purpose of this study is to determine antibiotic resistance patterns and detect the presence ofS. typhi IncHI1 plasmid genes in Jayapura.The method of study was the laboratory analytic where ninety two samples were culture tested and S. typhi identified was tested using disc diffusion method of Kirby Bouer to see any resistance toward 18 antibiotics. Plasmids of S. typhi then were extracted using method of Kado-Liu. To ensure the presence of S. typhi DNA, the testof flagellin gen detection had been done using Nested PCR followed by gene Inchi1 S. typhi detection. The results showed that among 8 isolates of S. typhi, there has been a Multi Drug Resistant (MDR) of 7 samples (87.5%) with varying resistance patterns where the most resistant antibiotics are Amoxicillin 100% (8 isolates), Cefazolin 75% (6 isolates), Ampicillin 75% (6 isolates), Trimethoprim–Sulfamethoksazol 62.5% (5 isolates), Amikacin 62.5% (5 isolates), Gentamicin 50% (4 isolates) and Ampisillin–Sulbactam 50% (4 isolates). The most sensitive antibiotics is Meropenem is 87.5% (7 isolates). PCR test results showed that there were no genes of IncHI1 S. typhi in Jayapura.
Key words:Â Salmonella typhi, gen IncHI1, antibiotic resistance, Jayapura.
Downloads
References
Amra, R. 2009. Deteksi antibodi IgM terhadap lipopolisakarida Salmonella typhi isolat Makassar dengan teknik lateral flow pada penderita demam tifoid di Jayapura, Makassar.
Anggeraini, A.S. 2013. Analisis mutasi gen CATp pada bakteri Salmonella typhi yang resisten terhadap kloramfenikol. [Tesis]. Universitas Hasanudin, Makasar.
Cita, Y.P. 2011. Bakteri Salmonella typhi dan demam tifoid. Jurnal Kesehatan Masyarakat. 6(1): 42–46.
Haque, A. 2006. catP gene. Sequencing of catP gene. Personal Communication.
Haque, A., A. Haque, Y. Sarwar, A. Ali, S. Bashir, A. Tariq and M. Mohsin. 2005. Identifikasi of drug resistence genes in clinical isolate of Salmonella typhi for development of diagnostic multiplex PCR. Pak J. Med. Sci. 21(4): 402–407.
Hardjono, T. Esa, dan Nurhayana. 2007. Kumpulan penyakit infeksi dan tes kultur sensitivitas kuman serta upaya pengendaliannya. Cahya Dinar Rucitra. Makassar.
Hatta, M. 2005 Pendekatan biologi molekuler dan imunologi di bidang penyakit infeksi dalam era globalisasi dan peluang bagi ilmuwan Indonesia. Suplement. Bagian Ilmu Mikrobiologi FK, Universitas Hasanuddin. Makassar.
Hatta, M., and H.L. Smits. 2007. Detection of Salmonella typhi by nested polymerase chain reaction in blood, urine, and stool samples. J Trop Med Hyg. 76(1): 139–143.
Hielda, 2009. Analisis gen catP yang manyandi resistensi terhadap kloramfenikol pada Salmonella typhi isolat Jayapura dengan teknik PCR. Makassar.
Indradewi A, F. 2007. Deteksi DNA plasmid pada Lactobacillus sp dengan metode elektroforesis gel agarosa. Warta Wiptek. 14(2): 67–69.
Irma, S., and A. Juniarti. 2010. Sensitivitas Salmonella typhi terhadap kloramfenikol dan seftriakson di RSUD Dr. Soetomo Surabaya dan di RSUD Dr. Saiful Anwar Malang tahun 2008-2009. Departemen Mikrobiologi Fakultas Kedokteran Universitas Muhammadiyah.
Mulyana, Y. 2008. Sensitivitas Salmonella Sp. penyebab demam tifoid terhadap beberapa antibiotik di Rumah Sakit Imanuel Bandung. [Makalah]. Bagian Mikrobiologi FK Universitas Padjajaran, Bandung.
Musnelina, L., A.E. Afdhal, dan A. Gani. 2004. Pola pemberian antibiotika pengobatan demam tifoid anak di Rumah Sakit Fatmawati Jakarta tahun 2001-2002. Makara Kesehatan. 8(1): 27–31.
Nurmala, I.G.N. Virgiandhy, Andriani, dan D.F. Liana. 2015. Resistensi dan sensitivitas bakteri terhadap antibiotik di RSU dr. Soedarso Pontianak tahun 2011-2013. JKI. 3(1): 21–28.
Nurtjahyani, D. 2007. Studi biologi molekuler resistensi Salmonella typhi terhadap cloramfenikol. Post Graduate. Universitas Airlangga. Surabaya.
Phan, M.-D. 2009. Analysis of IncHI1 plasmids in Salmonella enterica serovar Typhi. Dissertation Ph.D. Darwin College. University of Cambridge.
Riskayati, M. Hatta, R. Agus. 2012. Detection of the presence of Inchi 1 Plasmid Multilocus in Salmonella typhi against drug-resistant typhoid in Makassar. Pascasarjana. 23(1): 33–39.
Sitasiwi, A.J., W.T. Artama, A. Budiyanto, dan E. Dharmana. 2015. Kloning gen wingless-type MMTV integration site family member 4 (Wnt4) mencit Swiss Webster untuk penyediaan antigen baru dalam imunokontrasepsi. Prosiding Seminar Ilmiah PBBMI. UGM (Yogyakarta), 7 Maret 2015, Hal.: 11–23.
Thalheim, T., A. Vollmer, R.-U. Ebert, R. Kühne and G. Schüürmann. 2010. Tautomer identification and tautomer structure generation based on the InChI code. J. Chem. Inf. Model. 50(7): 1223–1232.
Wain, J., L.T.D. Nga, C. Kidgell, K. James, S. Fortune, T.S. Diep, T. Ali, P.Ó Gaora, C. Parry, J. Parkhill, J. Farrar, N.J. White, and G. Dougan. 2003. Molecular analysis of incHI1 antimicrobial resistance plasmids from Salmonella serovar typhi strains associated with typhoid fever. Antimicrob Agents Chemother. 47(9): 2732–2739.
Widodo, D. 2007. Demam tifoid: Ilmu penyakit dalam. Edisi IV. Fakultas Kedokteran Universitas Indonesia. Jakarta. pp: 1752–175.
Musnelina, L., A.F. Afdhal, A. Gani, dan P. Andayani. 2004. Analisis efektivitas biaya pengobatan demam tifoid anak menggunakan kloramfenikol dan seftriakson di Rumah Sakit Fatmawati Jakarta tahun 2001–2002. Makara Kesehatan. 8(2): 59–64.
