Toksisitas Isolat Lokal Bacillus thuringiensis (H-14) dan Isolat Sandi 18 Serta Lama Efektivitasnya di dalam Air terhadap Larva Nyamuk Anopheles dan Culex
DOI:
https://doi.org/10.31957/jbp.557Abstract
The research of toxicity local isolate Bacillus thurigiensis (H-14) and 18 codeword isolate along the efectivity on the water concerning to the Culex and Anopheles. The research aim to acquainted about the toxicity of B. thuringiensis isolate H-14 local and 18 codeword isolate along of the toxicity on the water concerning to the Culex and Anopheles. The methode is laboratory experiment wich used complete randome, analysis the data to wich BNJ test and 0.05% confidence standart. The result shows  that isolate of local B. huringiensis H-14 and coderword 18 toxic concerning to Culex and Anopheles B.thurngiensis H-14   0.06 ml concentrate, 0.08 ml  concentrate and 0.1 of effective of the Anopheles death until 6 day, and 0.1 ml effective concentrate show the Culex death until 7 day.Isolate 18 in 0.08 concentrate and 0.1 effective of  Culex  until 5 day and 0.1 ml concentrate 0.1 ml effective of the Anopheles until 7 day. Analysis varians shows the different act control concentrate  in the other side, the act of concentrate isnt different significant.Â
Key words: Bacillus thuringiensis, Local isolate, toxicity, Anopheles and Culex larva.
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References
Anonim. 2004a. The Microbial Word. Bacillus thuringiensis File://AB-t.htm.
Anonim. 2004b. Bacillus thuringiensis Pesticide fact Sheet. Prepared for the U.S. Departement of Agriculture Forest Service by information Venture. Inc. Mail US at: Customer- Service @ Information. Com-Http://Info Venture Come-hlth.
Blondine, Ch.P., Widiastuti, dan Widiarti. 1995. Uji Coba Bacillus thuringiensis H-14 Terhadap Jentik Nyamuk Anopheles barbirotris di Laboratorium dan lapangan. Bul.Pen. Kesehat. 23(1): 39-44.
Blondine, Ch.P., Rendro & Sukarno. 2000. Pengendalian Jentik Nyamuk Vektor Demam Berdarah, Malaria dan Filariasis Menggunakan Strain Lokal Bacillus thuringiensis H-14. Bul.Pen. Kesehat. 27(1): 283-286.
De Bach, P. 1974. Biological Control of Coccids by Introduced Natural Enemis. Plenum, New York. pp: 165- 193.
De Barjac, H. dan J. Bonnefoi. 1968. Essay Classification Biochemical the Bacillus du Type Bacillus thuringiensis Microbial Control of masquito pest and deseases. Acad. Press, London. p. 35-39.
Dubois, N.R. & F.B. Lewis. 1981. What is Bacillus thuringiensis. Journal. Arboricul. 7(9): 232-240.
Hadioetomo, R.S., dan I. Rusmana. 1996. Isolasi Bacillus thuringiensis Berl. Dari Peternakan Ulat Sutra dan Toksisitasnya Terhadap Larva Crocidolomia binotalis Zell, dan Spodoptera. Jurnal Hayati. 1(1): 21-23.
Hofte, H. dan R. Whiteley. 1989. Insecticidal Crystal Protein of Bacillus thuringiensis. Microbial. Rev. 53(2):242-255.
Huffakaer, C.B., dan P.S.Messenger. 1989. The Concept and Significance of Natural Control. In. Biological Control of Insect Pest and Weeds.Academic Prees, Toronto, Canada. Pp 12- 28.
Ignofo, C.M. dan C.F. Anderson.1979. Bioinsecticides. Microbial Technology. Academic Prees, New York.
Kasman, S., S. Andrew, dan N. Rabecca. 1998. Display of a Biologically Active Bacillus thuringiensis. Applied and Enviromental Microbiology. 64(8): 2995-3003.
Krieg, A. 1961. The Genus Bacillus Insect Pathogens. The Prokaryotes. Vol. II. Springer-Verlag, Berlin, Heidelberg, New York. 1741-1748
Kurniasari. 1994. Patogenitas Bacillus thuringiensis asal Indonesia terhadap larva Crocidolomia binotalis (Zeil) dan Spodoptera litura. Lap. Pen. IPB- Bogor. hal. 1-6.
Luthy, P. 1980. Insecticydal Toxin of Bacillus thuringiensis. Lett. 8: 1-7.
Mangoendihardjo. 1989. Teori dan Praktek Pengendalian Biologi. Edisi 1. Penerbit Universitas Indonesia (UI- Prees), Jakarta. hal. 13 – 70.
Munif, A. 1997. Pengaruh Bacillus thuringiensis H-14 Formula Tepung Pada Berbagai Instar Larva Nyamuk Aedes aegypti di Laboratorium. Cermin Dunia Kedokteran. (144): 78 – 91.
Nadrawati, J.Situmorang, & Mahrub. 1994. Isolasi Bacillus thuringiensis di Propinsi Daerah Istimewa Yogyakarta dan Uji Patogenitasnya Terhadap Spodoptera litura (Fabricus) dan Plutella xylostella Curt. BPPS-UGM.7(1b):3-5.
Norris, P. 1971. The Protein Crystal Toxin of Bacillus thuringiensis. Microbial Control of Insect and Mites. Academic Prees, London, hal 229-246.
Novianti, M. 1999. Pemanfaatan antigen H untuk identifikasi Bacillus thuringiensis. Lap. Pen. IPB,Bogor. hal 1-12.
Oktavina, D.M. 1999. Stabilitas Beberapa Formulasi Bioinsektisida Bacillus thuringiensis Subsp Aizawai. Laporan Penelitian IPB, Bogor. hal 1-6
Permatasari, U. 1998. Kinerja Bacillus thuringiensis dalam Medium Glukosa-Mineral dengan Dua Macam Sumber Nitrogen Yang Berbeda. Laporan Penelitian IPB, Bogor.hal 7-8.
Pramatha, R.B. 1994. Expression of parasporal crystasl protein (-endotoksin) gen() of Bacillus thuringiensis in sporogenic. J.Biosci.19(2):145-153.
Rukmono. 1991. Perkembangan baru dalam Pemberantasan Malaria. Maj. Kedok. 41: 189-193.
Santosa, dan H. Widyastuti. 1996. Toksisitas Beberapa Isolat Bacillus thuringiensis Terhadap Penggerek Buah Kakao dan karakteristik Gen Cry-nya. Jurnal Menara Perkebunan 64:123-132.
Lantang, 2005. The toxixity of Bacillus thuringiensis Isilates From several soil Habitat On The Some Area In Papua Province At Mosquito Larvae Of Anopheles farauti Laveran And several Its Characteristics. Jurnal Porsimapas. 11: 78 – 99.
Steel, R.G. dan J.H. Torrie. 1991. Prinsip dan Prosedur Statistika. PT. Gramedia Utama, Jakarta.
Subiantoro. 1997. Pertumbuhan Isolat Bacillus thuringiensis Pada Media Air Kelapa dan Uji Patogenitasnya Terhadap Jentik Nyamuk Vektor. Bul. Pen. Kesehat.26(2):95-102.
Wardhani, T. 1996. Bacillus thuringiensis Berliner Alternatif Pestisida Mikroba Untuk Mengendalikan Hama Moruca testulalis Geyer Pada Tanaman Kacang Hijau . Jur. Ilmiah Widya Gama 1(4):101-106.
Widiastuti, Widiarti, dan Sustriayu. 1997. Efikasi Bacillus thuringiensis H-14 (Vectobac G) Terhadap Jentik Nyamuk Anopheles barbirotris di laboratorium. Bul. Pen. Kes. 14(5): 12–19.
