Isolasi dan Aplikasi Fungi Entomopatogen dari Larva Nyamuk Aedes aegypti L.

Wuri Artikasari, Emantis Rosa, Bambang Irawan

Abstract


DHF (Dengue Hemorrhagic Fever) is a serious problem in Indonesia. DHF disease control has been applied so far, one of which is the use of larvacide temephos (abate). However larvacide is a chemical insecticide that has a negative impact on human health and causes resistance. Therefore in this research, biological control is carried out by utilizing entomopathogenic fungi as a larvacide. The purpose of this study was to determine the effectiveness of entomopathogenic fungi isolated from Ae aegypti larvae. Against the death of Ae. Aegypti mosquito larvae with the moist chamber method. This study used a completely randomized design (CRD) with a factorial pattern and performed two repetitions. Factor A is a type of fungi with 3 levels, namely A1: Aspergillus sp1, A2: Aspergillus sp2, and A3: Syncephalastrum sp. Factor B is a dilution with 7 levels, namely B0: Control, B1: 100 (without dilution), B2: 10-1, B3: 10-2, B4: 10-3, B5: 10-4, B6: 10-5 with every treatment was applied in 2 repetitions. Observations were made 24 hours after treatment for 3 days. Data were analyzed using analysis of variances (anova) and continued with the Least Significant Difference Test (LSD) at 5%. The results indicate that fungi isolates are the most effective in killing Ae. Aegypti mosquito larvae is Aspergillus sp1 and Aspergillus sp2 on the treatment of spores without dilution.

Key words: DHF; larvacide; Aedes aegypti; Entomopathogenic Fungi.

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References


Barnett, H.L., and B.B. Hunter. 1998. Illustrated genera of imperfect fungi. 4th Edition. Mac.Millian Publishing Company, New York.

Depkes RI. 2016. Pencegahan dan pemberantasan demam berdarah dengue di Indonesia. Depkes RI. Direktorat Jenderal Pengendalian Penyakit dan Penyehatan Lingkungan. Jakarta.

Freimoser, F.M., C.A. Jakob, M. Aebi, and Tuor. 1999. The MTT [3-(4,5- Dimethylthiazol-2-yl)-2,5-Diphenyl tetrazolium Bromide] assay is a fast and reliable method for colorimetric determination of fungal cell densities. Applied and Environmental Microbiology. 65(8): 3727-3729.

Gabriel, B.P., dan Riyanto. 1989. Metarhizium anisopliae taksonomi, patologi, produksi dan aplikasinya. Proyek Pengembangan Perlindungan Tanaman Perkebunan. Direktorat Perlindungan Tanaman Perkebunan. Departemen Pertanian. Jakarta.

Hadi, U.K., S. Soviana, dan D.D. Gunandini. 2012. Aktivitas nokturnal vektor demam berdarah dengue di beberapa daerah di Indonesia. Jurnal Entomologi Indonesia. 9(1): 1-6.

Hamid, S., F. Halouane, F.Z. Bisaad, dan F. Benzina. 2013. Study about the fffect of Beauveria bassiana on the aquatic stages of Culex pipiens. International Journal of Biotechnology and Research. 3: 31-42.

Handayani, T. dan S. Purwantisari. 2015. Isolation and identification of mold contaminants mushroom growing medium (bag lag) and their cellulolytic performance test. Jurnal Sains dan Matematika. 23: 55-58.

Herdatiarni, F., H. Toto, dan R. Rina. 2014. Eksplorasi cendawan entomopatogen Beauveria sp. Menggunakan serangga umpan pada komoditas jagung, tomat dan wortel organik di Batu, Malang. Jurnal HPT. 1(3): 2338–4336.

Kaur, S.P., R. Rao, dan S. Nanda. 2011. Amoxicillin: a broad spectrum antibiotic. International Journal of Pharmacy and Pharmaceutical Sciences. 3(3): 30–37.

Maharani, S.A., F. Rohman, dan S.E. Rahayu. 2016. Uji efektifitas jamur entomopatogen Beauveria bassiana Balsamo dan Verticillium lecanii (Zimmerman) Viegas terhadap mortalitas Helopeltis antonii Signoret. http://karyailmiah.um.ac.id.php/biologi/article. Diakses pada tanggal 2 Oktober 2019.

Masyitah, I., F.S. Sitepu, dan S. Irda. 2017. Potensi jamur entomopatogen untuk mengendalikan ulat grayak Spodoptera litura F. pada tanaman tembakau in vivo. Jurnal Agroekoteknologi. 5(3): 484-493.

Prayogo, Y. 2011. Sinergisme cendawan entomopatogen Lecanicillium lecanii dengan insektisida nabati untuk meningkatkan efikasi pengendalian telur kepik coklat Riptortus linearis pada kedelai. Jurnal HPT Tropika. 11(2): 166-177.

Ratledge, C. 1994. Biochemistry of microbial degradation. Kluwer Academic, Publishers. London.

Schuster, E., N. Dunn-Coleman, J. Frisvad, and P. Van Dijck. 2002. On the safety of Aspergillus niger–A review. Appl. Microbiol. Biotechnol. 59: 426-435.

Sandhu, S.S., A.K. Sharma, V. Beniwal, G. Goel, P. Batra, A. Kumar, S. Jaglan and S. Malhotra. 2012. Myco-biocontrol of insect pests: Factors involved, mechanism andregulation. Journal of Pathogens. 2012: 1-11.

Septiana, E. 2015. Jamur entomopatogen: Potensi dan tantangan sebagai insektisida alami terhadap serangga perusak tanaman dan vektor penyakit manusia. Biotrends. 1(1): 28-31.

Setyowati, E.A. 2013. Biologi nyamuk Aedes aegypti sebagai vektor demam berdarah dengue. Universitas Jenderal Soedirman. Purwokerto.

Sudarma, I.M., dan D.N. Suprapta. 2011. Potensi jamur antagonis yang berasal dari habitat tanaman pisang dengan dan tanpa gejala layu Fusarium untuk mengendalikan Fusarium oxysporum f.sp. cubense secara in vitro. [Skripsi]. Universitas Udayana. Bali.

Supriyadi, D., F. Pasaru dan I. Lakani. 2017. Efikasi cendawan Aspergillus sp terhadap hama penghisap buah kakao Helopeltis sp. (Hemiptera: Miridae) pada tanaman kakao. J. Agrotekbis. 5(3): 300-307.

Venkatasubbaiah, P., and K.M. Safeeulla. 1984. Aspergillus niger for biological control of Rhizoctonia solani on coffee seedling. Trop. Pest. Management. 30(4): 401-406.




DOI: https://doi.org/10.31957/jbp.833

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