Identifikasi Genetik Udang Mantis Dengan Pendekatan DNA Barcoding Gen Sitokrom Oksidase 1 (CO1)

Penulis

  • Muhammad Dailami Program Studi Budidaya Perairan, Departemen Manajemen Sumberdaya Perikanan dan Kelautan, FPIK Universitas Brawijaya
  • Dandi Saleky Program Studi Manajemen Sumberdaya Perairan, Jurusan Manajemen Sumberdaya Perairan, Faperta Universitas Musamus
  • Abdul Hamid A. Toha Program Studi Manajemen Sumberdaya Perairan, Jurusan Perikanan, FPIK Universitas Papua
  • Lalu Panji Imam Agamawan Program Studi Ilmu Perikanan, Jurusan Ilmu Kelautan dan Perikanan, FMIPA Universitas Cenderawasih

DOI:

https://doi.org/10.31957/acr.v5i1.2269

Abstrak

Mantis shrimp is one of the marine organisms that has a very abundant diversity of species. The similarity of morphology of this group make it difficult to identify morphologically. Therefore, identification was carried out using a DNA barcoding approach. The purpose of this study was to identify the mantis shrimp species from Manokwari waters using molecular techniques. The CO1 gene fragments were amplified by using LCO and HCO primers. The DNA sequences obtained were analyzed for homology, phylogeny, and genetic distance using MEGA X software. A total of 537 base pairs of CO1 gene fragments of mantis shrimp from Manokwari were obtained. The results of the homology analysis on GenBank, show the highest similarity of 91.18% with sequences from the genus Gonodactylus. Analysis of phylogenetic tree and genetic distance showed the same results and supported each other with homology analysis on GenBank data.

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Referensi

Boratyn, G. M., Camacho, C., Cooper, P. S., Coulouris, G., Fong, A., Ma, N., … Zaretskaya, I. 2013. BLAST: a more efficient report with usability improvements. Nucleic Acids Research, 41(W1), W29–W33. https://doi.org/10.1093/nar/gkt282

Camacho C., Coulouris G., Avagyan V., Ma N., Papadopoulos J., Bealer K., & Madden T.L. (2008) "BLAST+: architecture and applications." BMC Bioinformatics 10:421. PubMed

Dailami, M., Santi, D., Murtihapsari, ., Abubakar, H., & Toha, A. H. A. 2018. Genetic analisys of cytochrome oxidase sub unit 1 gene fragment from Cirrhilabrus cf. ryukyuensis (Labridae) from Cenderawasih Bay and Raja Ampat. Jurnal Iktiologi Indonesia, 18(3), 209. https://doi.org/10.32491/jii.v18i3.347

Dailami, M., Toha, A.H.A., Lapadi, I. and Kilawati, Y. 2021a. Genetic characteristics of lobster Panulirus versicolor (Latreille, 1804) from bird’s head seascape-Papua based on cytochrome oxidase subunit 1 (COI) gene. In IOP Conference Series: Earth and Environmental Science (Vol. 743, No. 1, p. 012020). IOP Publishing. Https://dx.doi.org/10.1088/1755-1315/743/1/012020

Dailami, M., Widyawati, Y. and Toha, A.H.A. 2021b. Genetic Identification of Anchovy from Cenderawasih Bay using DNA Barcoding Approach. Musamus Fisheries and Marine Journal, pp.154-166. https://doi.org/10.35724/mfmj.v3i2.3521

Folmer, O., Black M, Hoeh W, Lutz R, Vrijenhoek R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol. 1994;3:294–9.

Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547–1549. https://doi.org/10.1093/molbev/msy096

Lutfi, Abubakar, H., Manaf, M., Lapadi, I., & Dailami, M. 201). Genetic Identification of Aplocheilus Panchax from the Waters of West Papua Using Molecular Approach for Preventing the Spread of Malaria. Indian Journal of Public Health Research & Development, 10(10), 1348–1353. https://doi.org/10.5958/0976-5506.2019.03022.5

Nei, M., & Kumar, S. 2000. Molecular Evolution and Phylogenetics. Oxford University Press.

Patek, S. N. & Caldwell, R. L. (2005). "Extreme impact and cavitation forces of a biological hammer: strike forces of the peacock mantis shrimp". Journal of Experimental Biology. 208 (19): 3655–3664. doi:10.1242/jeb.01831

Ratnasingham, S., & Hebert, P. D. N. 2007. The Barcode of Life Data System BOLD : Molecular Ecology Notes, 2007(2007), 1–10. https://doi.org/10.1111/j.1471-8286.2006.01678.x

Ratnasingham S, and Hebert PDN. 2013. A DNA-Based Registry for All Animal Species: The Barcode Index Number (BIN) System. PLoS ONE 8(8): e66213. DOI:10.1371/journal.pone.0066213

Thompson, J. D., Higgins, D. G., & Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22(22), 4673–4680. https://doi.org/10.1093/nar/22.22.4673

Toha, A. H. A., Dailami, M., Anwar, S., Setiawan, J. B., Jentewo, Y., Lapadi, I., … Madduppa, H. 2020. The genetic relationships and indo-pacific connectivity of whale sharks (Rhincodon typus) with particular reference to mitochondrial COI gene sequences from Cendrawasih bay, Papua, Indonesia. Biodiversitas, 21(5), 2159–2171. https://doi.org/10.13057/biodiv/d210544

Van Der Wal, C., Ahyong, S.T., Ho, S.Y.W., Lo, N. 2017. "The evolutionary history of Stomatopoda (Crustacea: Malacostraca) inferred from molecular data". PeerJ. 5: e3844. doi:10.7717/peerj.3844

WoRMS. 2022. Stomatopoda. Accessed at: https://www.marinespecies.org/aphia.php?p=taxdetails&id=14355 on 2022-06-26

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2022-06-26

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