Fotodegradasi, Uji pH dan Kandungan In Vivo Pigmen Klorofil Lamun Thalasia hemprichii
DOI:
https://doi.org/10.31957/acr.v1i2.932Abstrak
Pigmen klorofil memiliki peran penting dan besar terhadap berbagai sistem kehidupan di perairan laut, dan dalam aplikasinya telah banyak digunakan untuk berbagai produk makanan, minuman, farmasi, dan kosmetik, sehingga studi terkait stabilitasnya perlu dilakukan. Tujuan penelitian ini adalah mengetahui kandungan, pengaruh sinar, dan pengaruh pH terhadap pigmen klorofil Thalasia hemprichii. Metode penelitian yang digunakan adalah (1) pengukuran kandungan secara in vivo, (2) ekstraksi aseton, (3) iradiasi, dan (4) uji pH. Hasil penelitian menunjukkan bahwa purata kandungan klorofil Thalasia hemprichii pada kedalaman 1 m = 15,42 spad/cm2, kedalaman 2 m = 17,82 spad/cm2, dan kedalaman 3 m = 19,01 spad/cm2. Pengaruh sinar merah selama 7 jam iradiasi menyebabkan degradasi pigmen di daerah Qx = 26,77% dan pada daerah Qy = 29,05% per jam, sedangkan iradiasi cahaya polikromatik selama 7 jam menyebabkan degradasi pada daerah Qx = 52,81% dan pada daerah Qy = 54,80%. Hasil uji pH menunjukkan bahwa klorofil lamun Thalasia hemprichii bersifat stabil pada pH 7 (netral), mengalami degradasi pada pH bersifat asam dan basa. Konsentrasi pH bersifat basa memiliki serapan tinggi, yaitu pH 9 (Qx = 0,732798 dan Qy = 0,362952), pH 11 (Qx = 0,879036 dan Qy = 0,433446), dan pH 13 (Qx = 0,948732 dan Qy = 0,459035), sedangkan pH bersifat asam memiliki serapan yang kecil, yaitu pH 5 (Qx = 0,787641 dan Qy = 0,391379) dan pH 1 (Qx = 0,736968 dan Qy = 0,264192).
Kata Kunci:Â Fotodegradasi; pH; Kandungan in vivo;Â Klorofil;Â Thalasia hemprichiiÂ
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